NUCLEOTIDE-SEQUENCE EVIDENCE FOR RAPID GENOTYPIC SHIFTS IN THE BOVINE MITOCHONDRIAL-DNA D-LOOP
NUCLEOTIDE-SEQUENCE EVIDENCE FOR RAPID GENOTYPIC SHIFTS IN THE BOVINE MITOCHONDRIAL-DNA D-LOOP
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DOI：
10.1038/306400a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
HAUSWIRTH, WW
中科院分区：
文献类型：
--
作者：
OLIVO, PD;VANDEWALLE, MJ;HAUSWIRTH, WW
线粒体DNA（mtDNA）在其快速进化1和高水平的种内序列变异2方面是不寻常的。后者被认为与线粒体DNA严格的母系遗传有关，这有效地隔离了一个物种内积累突变和独立变异的线粒体基因库。3这一过程的一个基本但尚未解释的方面是，面对103至105的体细胞和种系线粒体DNA倍性，（参考文献4，5），由突变事件产生的个体变异mtDNA分子可以如此迅速地支配细胞内大量的mtDNA群体。为了帮助回答这个问题，我们在这里确定了14头母系相关的荷斯坦奶牛的全部或部分D环区的核苷酸序列。在这些动物的线粒体DNA中可以区分出四种不同的D-loop序列。一种解释是，多个线粒体基因型存在于母体生殖系统中，并且在卵子发生或早期发育过程中这些基因型之一的扩增或分离导致观察到的快速基因型转变。
Mitochondrial DNA (mtDNA) is unusual in its rapid rate of evolution1and high level of intraspecies sequence variation2. The latter is thought to be related to the strict maternal inheritance of mtDNA, which effectively isolates within a species mitochondrial gene pools that accumulate mutations and vary independently3A fundamental and as yet unexplained aspect of this process is how, in the face of somatic and germ-line mtDNA ploidy of 103to 105(refs 4, 5), individual variant mtDNA molecules resulting from mutational events can come to dominate the large intracellular mtDNA population so rapidly. To help answer this question, we have determined here the nucleotide sequence of all or part of the D-loop region in 14 maternally related Holstein cows. Fourdifferent D-loop sequences can be distinguished in the mtDNA of these animals. One explanation is that multiple mitochondrial genotypes existed in the maternal germ tine and that expansion or segregation of one of these genotypes during oogenesis or early development led to the rapid genotypic shifts observed.