REEXPRESSION OF THE INTERMEDIATE FILAMENT NESTIN IN REACTIVE ASTROCYTES
REEXPRESSION OF THE INTERMEDIATE FILAMENT NESTIN IN REACTIVE ASTROCYTES
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DOI：
10.1006/nbdi.1995.0008
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发表时间：
1995-04-01
影响因子：
6.1
通讯作者：
BRUSTLE, O
中科院分区：
文献类型：
--
作者：
LIN, RCS;MATESIC, DF;BRUSTLE, O
中间丝巢蛋白在发育中的中枢神经系统（CNS）的多能干细胞中高度表达。在神经元和神经胶质发生过程中，巢蛋白被细胞类型特异性中间丝（例如神经丝和胶质细胞酸性蛋白（GFAP））取代。在这项研究中，我们证明，巢蛋白的表达是重新诱导损伤的成人大脑中的反应性星形胶质细胞。缺血性和机械性损伤后，在损伤部位周围的GFAP阳性细胞中发现了强烈而持续的巢蛋白表达。在携带lacZ基因的转基因小鼠中的病变实验表明，巢蛋白在反应性星形胶质细胞中的上调是通过在CNS发育过程中控制巢蛋白表达的相同序列介导的。这些观察结果和最近的数据反应性星形胶质细胞中的神经胶质细胞和神经元标记抗原的共表达点之间的密切关系增殖星形胶质细胞和神经上皮前体细胞。
The intermediate filament nestin is highly expressed in multipotential stem cells of the developing central nervous system (CNS). During neuro- and gliogenesis, nestin is replaced by cell type-specific intermediate filaments, e.g. neurofilaments and glial fibrillary acidic protein (GFAP). In this study, we demonstrate that nestin expression is re-induced in reactive astrocytes in the lesioned adult brain. Following ischaemic and mechanical lesioning, a strong and sustained expression of nestin was noted in GFAP-positive cells surrounding the lesion site. Lesion experiments in transgenic mice carrying the lacZ gene under control of regulatory sequences from the nestin gene suggested that the upregulation of nestin in reactive astrocytes is mediated via the same sequences that control nestin expression during CNS development. These observations and recent data on the co-expression of glial and neuronal marker antigens in reactive astrocytes point to a close relationship between proliferating astrocytes and neuroepithelial precursor cells.