Axonal neurofilaments are resistant to calpain-mediated degradation in the WLD(S) mouse.
Axonal neurofilaments are resistant to calpain-mediated degradation in the WLD(S) mouse.
复制标题
WLD(S) 小鼠的轴突神经丝对钙蛋白酶介导的降解具有抵抗力。
DOI：
10.1097/00001756-199905140-00007
复制
发表时间：
1999
期刊：
影响因子：
1.7
通讯作者：
Glass,JD
中科院分区：
文献类型：
--
作者：
Bernier,B;Castejon,S;Culver,DG;Glass,JD
WLD S小鼠迟发性沃勒变性表型的生物学基础尚未阐明，尽管已知该特征是轴突固有的。先前的数据表明，来自WLD S的神经对蛋白水解降解具有相对抗性。我们研究了响应于加入钙激活蛋白酶m-钙蛋白酶的神经丝降解的时间过程，比较了来自WLD S和野生型小鼠的神经。在10分钟的体外蛋白酶解过程中，WLD S的神经丝降解始终比野生型小鼠的神经丝慢。在Western印迹上进行直接比较，在反应时间2、4和6分钟时神经丝免疫反应性具有统计学显著差异（p< 0.01）。这些结果表明，导致WLD S表型的突变可能会影响钙蛋白酶和神经丝之间的蛋白水解相互作用。
THE biological basis for the phenotype of delayed Wallerian degeneration in the WLD S mouse has yet to be elucidated, although it is known that the characteristic is intrinsic to the axon. Previous data suggested that nerves from the WLD S are relatively resistant to proteolytic degradation. We investigated the time-course of neurofilament degradation in response to addition of the calcium-activated protease m-calpain, comparing nerves from WLD S and wild-type mice. During 10 min of in vitro proteolysis, neurofilaments from the WLD S were consistently slower to degrade than were neurofilaments from wild-type mice. Direct comparisons were performed on Western blots, with statistically significant differences in neurofilament immunoreactivity at 2, 4, and 6 min of reaction time (p< 0.01). These findings suggest that the mutation leading to the WLD S phenotype may affect the proteolytic interaction between calpain and neurofilaments.