Mucin (Muc-1) expression is differentially regulated in uterine luminal and glandular epithelia of the baboon (Papio anubis)
Mucin (Muc-1) expression is differentially regulated in uterine luminal and glandular epithelia of the baboon (Papio anubis)
复制标题
DOI：
10.1095/biolreprod54.5.939
复制
发表时间：
1996-05-01
影响因子：
3.6
通讯作者：
Carson, DD
中科院分区：
文献类型：
--
作者：
HildPetito, S;Fazleabas, AT;Carson, DD
在许多物种中，顶细胞表面糖复合物的组织和组成的变化伴随着子宫腔上皮细胞从预感受态到感受态的转变。尽管这一过程具有生物学和临床意义，但很少有分子标记物作为子宫容受性的有用预测因子。最近在小鼠中的研究表明，跨膜粘蛋白糖蛋白Muc-1在大多数条件下在腔上皮的顶端表面大量表达，并且在接受子宫中总是减少。这些和其他观察结果提示粘蛋白具有抗粘连的作用，其功能是维持非接受性子宫状态。一个泛种Muc 1特异性抗体识别的肽基序保守的胞质结构域的Muc-1被用来检查的时间和空间表达的细胞相关的Muc-1在狒狒子宫在各种条件下，包括植入前和围植入期。MUC-1的表达不是由雌激素单独影响，但需要孕激素的作用。在暴露于类固醇的动物中，Muc-1在基底和功能区的上皮中以低至中等水平表达。在植入前期的表面上皮中检测到Muc-1的最高表达，即，直至第8天（第0天=排卵日），或在接受模拟该阶段的类固醇激素方案（14天雌激素引发，随后7天雌激素加孕酮）的卵巢切除动物中。持续暴露于两种激素，即，如在第10-12天或在雌激素引发后给予14天雌激素加孕酮治疗的卵巢切除狒狒中所见，导致表面上皮中Muc-1表达的显著降低;然而，腺上皮中的染色模式未被该治疗改变。Muc-1在感受前期表面上皮的表达与这些上皮中雌激素和孕酮受体的存在有关。MUC-1的表达减少，既不抗雌激素治疗在prereceptive阶段，也不antierythestin 1治疗通过到接受阶段。此外，持续的MUC-1表达在功能和基底上皮与表达的resistin受体。因此，Muc-1的表达似乎是胆固醇依赖性的，而不是雌激素依赖性的。它的结论是，Muc-1在表面上皮细胞的表达作为一个标记的prereceptive阶段在狒狒和损失的Muc-1从表面上皮细胞与生成的接受子宫状态。
Changes in the organization and composition of apical cell surface glycoconjugates accompany the transition of luminal epithelial cells from the prereceptive to the receptive state of the uterus in many species. In spite of the biological and clinical significance of this process, few molecular markers have arisen as useful predictors of uterine receptivity. Recent studies in mice demonstrate that the transmembrane mucin glycoprotein, Muc-1, is abundantly expressed at the apical surface of luminal epithelia under most conditions and is invariably reduced in receptive uteri. These and other observations have led to the suggestion that mucins serve an antiadhesive role and function to maintain a nonreceptive uterine state. A pan-species Muc 1-specific antibody recognizing a peptide motif conserved in the cytoplasmic domain of Muc-1 was used to examine the temporal and spatial expression of cell-associated Muc-1 in baboon uteri under a variety of conditions, including the pre- and periimplantation periods. Muc-1 expression was not driven by estrogen influences alone, but required progesterone action. In animals exposed to both steroids, Muc-1 was expressed at low to moderate levels in epithelia of the basalis and functionalis regions. The highest expression of Muc-1 was detected in surface epithelium of the preimplantation phase, i.e., up to Day 8 (Day 0 = day of ovulation), or in ovariectomized animals receiving a steroid hormone regime that mimicked this phase (14 days of estrogen priming followed by 7 days of estrogen plus progesterone). Continued exposure to both hormones, i.e., as seen at Days 10-12 or in ovariectomized baboons given 14 days of estrogen plus progesterone treatment after estrogen priming, resulted in marked reduction of Muc-1 expression in the surface epithelium; however, staining patterns in the glandular epithelium were unchanged by this treatment. The expression of Muc-1 on the surface epithelium during prereceptive phase was associated with the presence of both estrogen and progestin receptors in these epithelia. Muc-1 expression was reduced by neither antiestrogen treatment during the prereceptive stage nor antiprogestin 1 treatment through to the receptive phase. Furthermore, persistent Muc-1 expression in the functionalis and basalis epithelium correlated with expression of progestin receptors. Thus, Muc-1 expression appea red to be progesterone-dependent rather than estrogen-dependent. It is concluded that Muc-1 expression in surface epithelium serves as a marker of the prereceptive phase in the baboon and that loss of Muc-1 from surface epithelium correlates with generation of a receptive uterine state.