IgE expression pattern in lung: Relation to systemic IgE and asthma phenotypes
IgE expression pattern in lung: Relation to systemic IgE and asthma phenotypes
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DOI：
10.1016/j.jaci.2006.12.642
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发表时间：
2007-04-01
影响因子：
14.2
通讯作者：
Wenzel, Sally E.
中科院分区：
文献类型：
--
作者：
Balzar, Silvana;Strand, Matthew;Wenzel, Sally E.
背景：IgE 介导的反应会导致过敏和哮喘。关于组织 IgE 与全身 IgE、炎症或临床结果的关系知之甚少。目的：评估正常受试者和不同严重程度的哮喘受试者近端和远端肺中的局部 IgE 表达和细胞炎症，并将这些组织参数与全身 IgE 水平、特应性、肺功能和哮喘严重发作史相关联。方法： 对超过 90 名嗜酸性粒细胞 (SAeo(+)) 和非嗜酸性粒细胞 (SAeo(-)) 重度哮喘、轻度哮喘受试者和正常受试者进行了 IgE、IgE 受体信号放大亚型 (Fc epsilon RI beta) 以及肥大细胞、嗜酸性粒细胞和淋巴细胞标记物的免疫染色。比较了 IgE、Fc epsilon RI beta、细胞炎症、血清 IgE 和特应性的组织表达。使用回归模型来确定局部和全身 IgE 与肺功能和哮喘严重发作的关系。结果：肥大细胞结合的 IgE 存在于气道中，但在肺实质中不存在。尽管各组在全身/血清 IgE 和特应性方面相似，但局部/组织 IgE 在 SAeo(+) 中最高，并且与嗜酸性粒细胞和淋巴细胞相关 (r(s) = 0.52，P
Background: IgE-mediated responses contribute to allergy and asthma. Little is understood regarding the relationship of tissue IgE to systemic IgE, inflammation or clinical outcomes.Objectives: To evaluate local IgE expression and cellular inflammation in the proximal and distal lung of normal subjects and subjects with asthma of varying severity and relate those tissue parameters to systemic IgE levels, atopy, lung function, and history of severe exacerbations of asthma.Methods: Tissue from more than 90 subjects with eosinophilic (SAeo(+)) and noneosinophilic (SAeo(-)) severe asthma, mild asthma and normal subjects were immunostained for IgE, signal-amplifying isoform of IgE receptor (Fc epsilon RI beta) and markers of mast cells, eosinophils, and lymphocytes. Tissue expression of IgE, Fc epsilon RI beta, cellular inflammation, serum IgE, and atopy were compared. Regression models were used to determine the relationship of local and systemic IgE to lung function and severe exacerbations of asthma.Results: Mast cell-bound IgE was present along airways but absent in lung parenchyma. Although the groups were similar in systemic/serum IgE and atopy, local/tissue IgE was highest in SAeo(+) and correlated with eosinophils and lymphocytes (r(s) = 0.52, P