Role of opiate receptors in regulation of enkephalin stimulation of active sodium and chloride absorption.
Role of opiate receptors in regulation of enkephalin stimulation of active sodium and chloride absorption.
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阿片受体在调节脑啡肽刺激活性钠和氯吸收中的作用。
DOI：
10.1152/ajpgi.1984.247.4.g432
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Dobbins,JW
中科院分区：
文献类型：
--
作者：
Binder,HJ;Laurenson,JP;Dobbins,JW
阿片肽刺激兔回肠主动钠和氯吸收的机制尚不清楚。由于血管活性肠肽（VIP）调节电解质转运是由特定的膜受体介导的，这些实验试图确定阿片肽的膜受体是否存在于兔回肠肠上皮细胞。虽然我们发现特异性结合的放射性标记的阿片肽的匀浆的大脑和回肠肌间神经丛和125 I-VIP回肠肠上皮细胞，特异性结合的放射性标记的阿片肽的回肠肠上皮细胞或其匀浆未被确定。在平行研究中，河豚毒素，一种神经传递抑制剂，没有改变VIP刺激的Cl分泌，但抑制D-Ala 2-甲硫氨酸-脑啡酰胺诱导的电解质吸收。这些研究表明，阿片肽刺激活性钠和氯吸收介导的一个身份不明的中间激动剂。
The mechanism of opioid peptide stimulation of active Na and Cl absorption in rabbit ileum is not known. Since vasoactive intestinal peptide (VIP) modulation of electrolyte transport is mediated by specific membrane receptors, these experiments sought to determine whether membrane receptors for opioid peptides are present on rabbit ileal enterocytes. Although we found specific binding both of radiolabeled opioid peptides to homogenates of cerebrum and ileal myenteric plexi and of 125I-VIP to ileal enterocytes, specific binding of radiolabeled opioid peptides to either ileal enterocytes or their homogenates was not identified. In parallel studies, tetrodotoxin, an inhibitor of neurotransmission, did not alter VIP-stimulation of Cl secretion but inhibited D-Ala2-methionine-enkephalinamide-induced electrolyte absorption. These studies suggest that opioid peptide stimulation of active Na and Cl absorption is mediated by an unidentified intermediary agonist.