Tau associates with actin in differentiating PC12 cells
Tau associates with actin in differentiating PC12 cells
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DOI：
10.1096/fj.05-5206com
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发表时间：
2006-07
期刊：
影响因子：
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通讯作者：
Jiang-Zhou Yu;M. Rasenick
中科院分区：
文献类型：
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作者：
Jiang-Zhou Yu;M. Rasenick
微管相关蛋白tau可能参与细胞形态发生和轴突维持。除了微管，tau已经被证明在体外与肌动蛋白相互作用。在本研究中，研究了tau和肌动蛋白在PC12细胞中的相互作用。未经NGF处理时，未观察到tau与肌动蛋白的相互作用。在NGF刺激下，tau分布于细胞伸展末端，与肌动蛋白以不依赖微管的方式结合。F-肌动蛋白的破坏表明，在NGF诱导的tau重组和与肌动蛋白的结合中，F-肌动蛋白在细胞延伸末端的重新定位和组装是必要的。截短的tau-GFP(tau(1-186)-GFP，tau的N端)不与肌动蛋白结合。然而，tau23(174-352)-GFP(Tau23的羧基末端)确实与肌动蛋白结合，对NGF的需求消失。然而，NGF促进了tau23(174-352)-GFP与肌动蛋白的相互作用，并促进了细胞延伸末端的共定位。这表明tau的C-末端是与肌动蛋白结合所必需的，而tau的N-末端可能在这一过程中起着调节作用。推测了tau-肌动蛋白相互作用在神经突起生长中的可能作用-Yu，J.-Z.，Rasenick，M.M.Tau与肌动蛋白在PC12细胞分化中的作用。FASE B J.20,1452-1461(2006)
The microtubule‐associated protein tau may be involved in cell morphogenesis and axonal maintenance. In addition to microtubules, tau has been shown to interact with actin in vitro. In the present study interaction of tau and actin was investigated in PC12 cells. No interaction between tau and actin was observed without NGF treatment. Under NGF stimulation, tau distributed at ends of cellular extensions, where it associated with actin in a microtubule‐independent manner. F‐actin disruption revealed that relocalization and assembly of F‐actin at the ends of cellular extensions were necessary for NGF‐induced tau reorganization and association with actin. A truncated tau‐GFP (tau(1–186)‐GFP, N‐terminal of tau) did not associate with actin. However, tau23(174–352)‐GFP (carboxyl‐terminal of Tau23) did associate with actin and the requirement for NGF was lost. Nevertheless, NGF boosted tau23(174–352)‐GFP interaction with actin and promoted colocalization at the ends of cellular extensions. This suggests that the C‐terminal of tau is required for associating with actin and the tau N‐terminal may play a regulatory role in this process. A possible role for tau‐actin interaction in neurite outgrowth is postulated—Yu, J.‐Z., Rasenick, M. M. Tau associates with actin in differentiating PC12 cells. FASEB J. 20, 1452–1461 (2006)