Growth of microorganisms in an interfacially driven space bioreactor analog
Growth of microorganisms in an interfacially driven space bioreactor analog
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DOI：
10.1038/s41526-020-0101-4
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发表时间：
2020-04-08
期刊：
影响因子：
5.1
通讯作者：
Bonocora, Richard P.
中科院分区：
文献类型：
--
作者：
Adam, Joe A.;Gulati, Shreyash;Bonocora, Richard P.
微重力环境下的流体生物反应器可以采用密封和混合的替代方法。环形剪切液滴（RSD）是一种无容器混合装置，它在微重力下使用表面张力进行密封，并通过界面驱动流动进行混合。为了评估使用界面驱动流动装置（如RSD）作为生物反应器的可行性，在RSD的地面模拟物刀口表面粘度计（KEV）中分析了大肠杆菌的生长和重组蛋白的表达。结果表明，KEV能促进大肠杆菌的生长，且随着刀口转速的增加，大肠杆菌的生长速度呈对数增长，与地球上的标准生长方法（轨道激振器）相似。此外，KEV被证明能够支持重组蛋白在大肠杆菌中的表达，其水平与使用标准生长方法获得的水平相当。
Fluid bioreactors in microgravity environments may utilize alternative methods of containment and mixing. The ring-sheared drop (RSD) is a containerless mixing device which functions in microgravity using surface tension for containment and mixes through interfacially-driven flow. To assess the feasibility of using interfacially driven flow devices, such as the RSD, as bioreactors, Escherichia coli growth and recombinant protein expression were analyzed in a ground-based analog of the RSD called the knife edge surface viscometer (KEV). Results demonstrated that the KEV can facilitate the growth of E. coli and that growth rate increases logarithmically with increasing knife edge rotation rate, similar to the standard growth method on Earth (orbital shaker). Furthermore, the KEV was shown to be viable for supporting recombinant protein expression in E. coli at levels comparable to those achieved using standard growth methods.