Molecular cloning of the beta-subunit of the Na,K-ATPase in the brine shrimp, Artemia. The cDNA-derived amino acid sequence shows low homology with the beta-subunits of vertebrates except in the single transmembrane and the carboxy-terminal domains.
Molecular cloning of the beta-subunit of the Na,K-ATPase in the brine shrimp, Artemia. The cDNA-derived amino acid sequence shows low homology with the beta-subunits of vertebrates except in the single transmembrane and the carboxy-terminal domains.
复制标题
丰年虾 Na,K-ATP 酶 β 亚基的分子克隆。
DOI：
10.1016/0014-5793(90)81162-h
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
Hokin,LE
中科院分区：
文献类型：
--
作者：
Bhattacharyya,KK;Bergstrom,EE;Hokin,LE
编码丰年虾 Na,K-ATP 酶 β 亚基的 cDNA 已被克隆。其核苷酸序列和预测的氨基酸序列已确定。其氨基酸序列与鸡、狗、人、猪、大鼠、羊、鱼雷和爪蟾的氨基酸序列有相当大的差异。这并不完全出乎意料，因为丰年虾是一种“快时钟”生物，在 0.5 至 10 亿年前就与脊椎动物的前身分开了。然而，高度疏水的假定跨膜结构域和羧基末端结构域显示出相当大的保守性。贫血β亚基中相对较小程度的保守性应该提供有关该蛋白质功能意义的信息。
A cDNA encoding the β‐subunit of the Na,K‐ATPase of brine shrimp (Artemia) has been cloned. Its nucleotide sequence and predicted amino acid sequence have been determined. The amino acid sequence shows considerable divergence from that of chicken, dog, human, pig, rat, sheep,Torpedo, andXenopus. This is not entirely unexpected since brine shrimp is a ‘fast clock’ organism which diverged from the precursor of the vertebrates 0.5–1.0 billion years ago. However, a highly hydrophobic putative transmembrane domain and the carboxy‐terminal domain show considerable conservation. The relatively small degree of conservation in the β‐subunit ofAnemiashould provide information about the functional significance of this protein.