LINEAGE COMMITMENT OF HEMATOPOIETIC PROGENITOR CELLS IN DEVELOPING BLAST CELL COLONIES - INFLUENCE OF COLONY-STIMULATING FACTORS
LINEAGE COMMITMENT OF HEMATOPOIETIC PROGENITOR CELLS IN DEVELOPING BLAST CELL COLONIES - INFLUENCE OF COLONY-STIMULATING FACTORS
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DOI：
10.1073/pnas.88.24.11310
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发表时间：
1991-12-01
影响因子：
11.1
通讯作者：
METCALF, D
中科院分区：
文献类型：
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作者：
METCALF, D
在正常小鼠骨髓细胞的克隆培养物中，粒细胞、粒细胞-巨噬细胞或多能集落刺激因子（分别为 G-CSF、GM-CSF 或多能集落刺激因子）与干细胞因子 (SCF) 的组合与单独使用 SCF 相比，不会改变刺激发育的母细胞集落数量，但诱导其平均细胞含量增加高达 25 倍，平均祖细胞增加高达 6 倍内容。与仅用SCF刺激的集落相比，SCF加G-CSF对母细胞集落形成的共刺激并没有改变集落内不同类型的祖细胞的相对频率。然而，GM-CSF或多CSF与SCF的组合显着增加了粒细胞祖细胞的相对频率，对于多CSF，还显着增加了嗜酸性粒细胞祖细胞的相对频率。定型为不同谱系的祖细胞相对频率的这些变化支持了以下假设：造血调节因子具有一定能力诱导多能细胞后代的选择性谱系定型。
In clonal cultures of normal mouse marrow cells, combination of granulocyte, granulocyte-macrophage, or multipotential colony-stimulating factor (G-CSF, GM-CSF, or multi-CSF, respectively) with stem cell factor (SCF) did not alter the number of blast colonies stimulated to develop compared with SCF alone but induced an up to 25-fold increase in their mean cell content and an up to 6-fold increase in their mean progenitor cell content. Costimulation of blast colony formation by SCF plus G-CSF did not change the relative frequency of progenitor cells of different types within the colonies compared with colonies stimulated by SCF alone. However, combination of GM-CSF or multi-CSF with SCF significantly increased the relative frequency of granulocytic progenitors and, for multi-CSF, also of eosinophil progenitor cells. These changes in the relative frequencies of progenitor cells committed to the various lineages support the hypothesis that hemopoietic regulators have some ability to induce selective lineage commitment in the progeny of multipotential cells.