Stimulatory effect of bicarbonate on the glycolysis of Actinomyces viscosus and its biochemical mechanism.
Stimulatory effect of bicarbonate on the glycolysis of Actinomyces viscosus and its biochemical mechanism.
复制标题
碳酸氢盐对粘性放线菌糖酵解的刺激作用及其生化机制。
DOI：
10.1111/j.1399-302x.1992.tb00530.x
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发表时间：
1992
影响因子：
--
通讯作者：
Yamada,T
中科院分区：
文献类型：
--
作者：
Takahashi,N;Yamada,T
在厌氧条件下，研究了碳酸氢盐对4株人粘性放线菌产酸的影响。碳酸氢盐的产酸速度是不加碳酸氢盐的3-4倍。对细胞内糖酵解中间产物NAD和NADH的分析表明，当有碳酸氢盐存在时，细胞内NADH/NAD比率降低，3-磷酸甘油水平升高。此外，在碳酸氢盐存在的情况下，琥珀酸途径中的苹果酸脱氢酶和富马酸还原酶除了乳酸脱氢酶外，还可能作为NADH氧化酶发挥作用。这些观察表明，在碳酸氢盐存在的情况下，NAD可以有效地再生。因此，对FA的刺激。碳酸氢盐引起的粘性糖酵解被认为是由于3-磷酸甘油醛脱氢酶(G3PDH)被NADH水平降低而激活，因为NADH是该微生物中G3PDH的强烈抑制剂。
The effects of bicarbonate on acid production by 4 human strains ofActinomyces viscosuswere estimated under anaerobic conditions. The rate of acid production was accelerated by bicarbonate 3‐4‐times as much as that without bicarbonate. The analyses of intracellular glycolytic intermediates, NAD and NADH revealed a decrease in NADH:NAD ratio and an increase in the level of 3‐phosphoglycerate in the cells when bicarbonate was present. Furthermore, when bicarbonate was available, malate dehydrogenase and fumarate reductase in the succinate pathway were expected to function as NADH‐oxidizing enzymes in addition to lactate dehydrogenase. These observations indicate the efficient regeneration of NAD in the presence of bicarbonate. Thus, the stimulation ofA. viscosusglycolysis by bicarbonate was thought to stem from the activation of glyceraldehyde 3‐phosphate dehydrogenase (G3PDH) by the decrease in the level of NADH, because NADH was a strong inhibitor of G3PDH in this microorganism.