Microsatellite Variation in Two Subspecies of Cynomolgus Monkeys (Macaca fascicularis)
Microsatellite Variation in Two Subspecies of Cynomolgus Monkeys (Macaca fascicularis)
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DOI：
10.1002/ajp.21984
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发表时间：
2012-06
影响因子：
2.4
通讯作者：
Aixue Li;Zhaozeng Sun;L. Zeng;Ruisheng Li;Deqiang Kong;Yanbin Zhao;Jie‐ying Bai;Shuang Zhao;Shichen Shang;Yansheng Shi
中科院分区：
文献类型：
--
作者：
Aixue Li;Zhaozeng Sun;L. Zeng;Ruisheng Li;Deqiang Kong;Yanbin Zhao;Jie‐ying Bai;Shuang Zhao;Shichen Shang;Yansheng Shi
为估计食蟹猴两个亚种（Macacafascicularis fascicularis和M. F. aurea），从已有的微卫星标记中筛选出26个微卫星标记。17个标记在猴子的一个子集中表现出高多态性，并用于评估较大样本中的遗传多样性。对M. F. Aurea猴均显著高于M. F. fascicularis猴（P < 0.05），提示M. F.金猴的遗传变异程度高于猕猴。F.食蟹猴在食蟹猴的两个亚种之间也检测到等位基因分布的显著差异。私人等位基因仅限于M。F. fascicularis或M. F.在所选的17个基因座中均发现了金丝猴。这些私人等位基因可能允许区分食蟹猴的两个亚种。所选标记也可用于估计食蟹猴其他亚种的遗传变异。使用从本地或独立来源获得的额外动物进行进一步的工作对于更全面地了解这两个亚组之间的遗传差异将是重要的。Am. J. Primatol. 74：561-568，2012.© 2012 Wiley Periodicals，Inc.
To estimate the genetic variability of two subspecies of cynomolgus monkeys (Macaca fascicularis fascicularis and M. f. aurea) using microsatellite markers, 26 microsatellite markers were selected from previous reports. Seventeen markers showed high polymorphism in a subset of monkeys and were used for the assessment of genetic diversity in the larger sample. The effective number of alleles, the polymorphism information content (PIC) and the expected heterozygosity of M. f. aurea monkeys were all statistically significantly higher than those of M. f. fascicularis monkeys (P < 0.05), suggesting the M. f. aurea monkeys had a higher degree of genetic variation than the M. f. fascicularis monkeys. Substantial differences in allele distribution were also detected between the two subspecies of cynomolgus monkeys. Private alleles restricted to the M. f. fascicularis or the M. f. aurea monkeys were found throughout the selected 17 loci. These private alleles may allow the discrimination of the two subspecies of cynomolgus monkeys. The selected markers could also be used to estimate the genetic variation for other subspecies of cynomolgus monkeys. Further work using additional animals obtained from native or independent sources will be important for a more complete understanding of the genetic differences between these two subgroups. Am. J. Primatol. 74:561–568, 2012. © 2012 Wiley Periodicals, Inc.