Immunohistochemical Analysis of IA-2 Family of Protein Tyrosine Phosphatases in Rat Gastrointestinal Endocrine Cells
Immunohistochemical Analysis of IA-2 Family of Protein Tyrosine Phosphatases in Rat Gastrointestinal Endocrine Cells
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DOI：
10.1369/0022155412466872
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发表时间：
2013-02-01
影响因子：
3.2
通讯作者：
Torii, Seiji
中科院分区：
文献类型：
--
作者：
Gomi, Hiroshi;Kubota-Murata, Chisato;Torii, Seiji
胰岛相关蛋白-2（IA-2）和IA-2 β（也称为phogrin）是独特的神经内分泌特异性蛋白酪氨酸磷酸酶（PTP）。PTPs的IA-2家族最初从胰岛素瘤细胞中鉴定，并被发现是1型糖尿病的主要自身抗原。尽管其在神经和典型内分泌组织中表达，但关于PTPs的IA-2家族在胃肠内分泌细胞（GEC）中表达的数据有限。因此，我们用化学方法研究了PTPs IA-2家族在大鼠胃肠道中的表达。在胃中，IA-2和IA-2 β在分泌5-羟色胺、生长抑素和胆囊收缩素/胃泌素-1的GEC中表达。除了这些激素外，促胰液素、胃抑制多肽（也称为葡萄糖依赖性促胰岛素肽）、胰高血糖素样肽-1和胰高血糖素，但不与十二指肠GEC中的IA-2或IA-2 β共表达。分泌肠激素的胰岛细胞表达PTPs的IA-2家族。IA-2和IA-2 β的表达模式相当。这些结果表明PTPs的IA-2家族在大鼠GEC中以细胞类型特异性方式表达。PTPs的IA-2家族在胰腺-胃肠内分泌细胞和肠神经丛中的广泛表达表明它们通过神经内分泌信号网络对营养控制的系统性贡献。（J Histochem Cytochem 61：156-168，2013）
Islet-associated protein-2 (IA-2) and IA-2 beta (also known as phogrin) are unique neuroendocrine-specific protein tyrosine phosphatases (PTPs). The IA-2 family of PTPs was originally identified from insulinoma cells and discovered to be major autoantigens in type 1 diabetes. Despite its expression in the neural and canonical endocrine tissues, data on expression of the IA-2 family of PTPs in gastrointestinal endocrine cells (GECs) are limited. Therefore, we immunohistochemically investigated the expression of the IA-2 family of PTPs in the rat gastrointestinal tract. In the stomach, IA-2 and IA-2 beta were expressed in GECs that secrete serotonin, somatostatin, and cholecystokinin/gastrin-1. In addition to these hormones, secretin, gastric inhibitory polypeptide (also known as the glucose-dependent insulinotropic peptide), glucagon-like peptide-1, and glucagon, but not ghrelin were coexpressed with IA-2 or IA-2 beta in duodenal GECs. Pancreatic islet cells that secrete gut hormones expressed the IA-2 family of PTPs. The expression patterns of IA-2 and IA-2 beta were comparable. These results reveal that the IA-2 family of PTPs is expressed in a cell type-specific manner in rat GECs. The extensive expression of the IA-2 family of PTPs in pancreo-gastrointestinal endocrine cells and in the enteric plexus suggests their systemic contribution to nutritional control through a neuroendocrine signaling network. (J Histochem Cytochem 61: 156-168, 2013)