Low field nuclear magnetic sensing technology based on hydrogel-coated superparamagnetic particles
Low field nuclear magnetic sensing technology based on hydrogel-coated superparamagnetic particles
复制标题
基于水凝胶涂层超顺磁颗粒的低场核磁传感技术
DOI：
10.1016/j.aca.2019.10.013
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发表时间：
2020-01-15
影响因子：
6.2
通讯作者：
Yang, Shi-Ping
中科院分区：
文献类型：
--
作者：
Guo, Qi-Yue;Ren, Shu-Yue;Yang, Shi-Ping
基于超顺磁性纳米粒子，设计了一种通过核酸发夹杂交链式反应自组装的响应性聚丙烯酰胺水凝胶，并成功构建了一种通用的低场核磁共振传感平台。随着靶标的逐渐加入，磁性纳米颗粒表面的水凝胶涂层通过与适体的结合而被逐层打开，适体特异性地结合在磁性纳米颗粒上，导致磁性纳米颗粒不同程度的暴露，从而导致低场核磁共振信号的变化。该方法最初应用于三磷酸腺苷（ATP）的快速检测，并使用多氯联苯77（PCB77）验证了该方法的通用性。该方法具有快速、方便、成本低、操作简单、重复性好等优点。这种通用的方法可以通过替换适体来检测各种靶点，并且可能用于控制食品质量和体外快速检测癌细胞。(C)2019由Elsevier B.V.出版
Based on superparamagnetic nanoparticles, a responsive polyacrylamide hydrogel self-assembled by nucleic acid hairpin hybridization chain reaction was designed, and a universal low field nuclear magnetic resonance sensing platform was successfully constructed. As the target was gradually added, the hydrogel coating on the surface of the magnetic nanoparticle was opened layer by layer through binding with the aptamer, which specifically bonded thereto, causing different degrees of exposure of the magnetic nanoparticle, resulting in changes of low field nuclear magnetic resonance signals. This method was originally applied to the rapid detection of adenosine triphosphate (ATP), and the versatility of the method was verified using polychlorinated biphenyl 77 (PCB77). This method had the advantage of being fast, convenient, and low cost, and it can be easily operated with high repeatability. This universal method can detect a variety of targets by replacing aptamers and may be useful in controlling food quality and for rapidly detecting cancer cells in vitro. (C) 2019 Published by Elsevier B.V.