Glycosylation of acetylcholinesterase and butyrylcholinesterase changes as a function of the duration of Alzheimer's disease
Glycosylation of acetylcholinesterase and butyrylcholinesterase changes as a function of the duration of Alzheimer's disease
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DOI：
10.1002/jnr.10599
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发表时间：
2003-05-15
影响因子：
4.2
通讯作者：
Small, DH
中科院分区：
文献类型：
--
作者：
Sáez-Valero, J;Fodero, LR;Small, DH
阿尔茨海默病（AD）生化标志物的鉴定可能有助于该疾病的诊断。先前的研究表明，AD脑脊液（CSF）中Abeta（1-42）降低，tau和磷酸化tau增加。我们自己的研究已经确定了乙酰胆碱酯酶（Glyc-ACNE）和丁酰胆碱酯酶（Glyc-BuChE）的糖基化亚型在AD CSF中增加。Glyc-ACNE在APP（SW）Tg 2576转基因小鼠中在淀粉样斑块沉积之前增加，这表明Glyc-ACNE可能是AD的早期标志物。本研究的目的是确定在AD早期阶段CSF中Glyc-ACNE或Glyc-BuChE是否增加，并将这些标志物的水平与Abeta（1-42）、tau和磷酸化tau的水平进行比较。从106名非AD患者中获得腰椎CSF，其中包括15名轻度认知障碍（MCI）患者和102名可能患有AD的患者。与非神经系统疾病（NND）对照组相比，AD患者CSF中的Glyc-ACNE、tau和磷酸化tau显著增加。AD患者的Abeta（1-42）低于NND对照组。Glyc-ACNE或Glyc-BuChE水平与病程呈正相关。然而，tau、磷酸化tau或Abeta（1-42）的水平与疾病持续时间之间没有明确的相关性。结果表明，Glyc-ACNE和Glyc-BuChE不太可能是AD的早期标志物，尽管它们可能具有作为疾病进展标志物的价值。(C)2003 Wiley-Liss，Inc.
The identification of biochemical markers of Alzheimer's disease (AD) may help in the diagnosis of the disease. Previous studies have shown that Abeta(1-42) is decreased, and tau and phospho-tau are increased in AD cerebrospinal fluid (CSF). Our own studies have identified glycosylated isoforms of acetylcholinesterase (Glyc-ACNE) and butyrylcholinesterase (Glyc-BuChE) that are increased in AD CSF. Glyc-ACNE is increased in APP (SW) Tg2576 transgenic mice prior to amyloid plaque deposition, which suggests that Glyc-ACNE may be an early marker of AD. The aim of this study was to determine whether Glyc-ACNE or Glyc-BuChE is increased in CSF at early stages of AD and to compare the levels of these markers with those of Abeta(1-42), tau and phospho-tau. Lumbar CSF was obtained ante mortem from 106 non-AD patients, including 15 patients with mild cognitive impairment (MCI), and 102 patients with probable AD. Glyc-ACNE, tau and phospho-tau were significantly increased in the CSF of AD patients compared to nonneurological disease (NND) controls. Abeta(1-42) was lower in the AD patients than in NND controls. A positive correlation was found between the levels of Glyc-ACNE or Glyc-BuChE and disease duration. However, there was no clear correlation between the levels of tau, phosphotau or Abeta(1-42) and disease duration. The results suggest that Glyc-ACNE and Glyc-BuChE are unlikely to be early markers of AD, although they may have value as markers of disease progression. (C) 2003 Wiley-Liss, inc.