In Vivo Evaluation of Cartilage Regenerative Effects of CCN2 Protein
In Vivo Evaluation of Cartilage Regenerative Effects of CCN2 Protein
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CCN2蛋白软骨再生作用的体内评价
DOI：
10.1007/978-1-4939-6430-7_25
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Kubota S and Takigawa M
中科院分区：
文献类型：
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作者：
Nishida T;Kubota S and Takigawa M
CCN家族蛋白2/结缔组织生长因子（CCN 2/CTGF）是一种独特的生长因子，在体外能促进关节软骨细胞的增殖和分化，但不促进关节软骨细胞的肥大。基于这些发现，我们先前评估了重组CCN 2蛋白（rCCN 2）的软骨再生作用，通过使用单碘乙酸（MIA）注射到大鼠关节腔和在体内形成大鼠关节软骨的全层缺损，我们的结果表明CCN 2在关节软骨再生中的效用。本章提供了应用这两种动物模型评估CCN 2或其衍生物的软骨再生作用的有用提示。
CCN family protein 2/connective tissue growth factor (CCN2/CTGF) is a unique growth factor that promotes the proliferation and differentiation, but not the hypertrophy of articular chondrocytes in vitro. Based on these findings, we previously evaluated the cartilage-regenerative effects of recombinant CCN2 protein (rCCN2) by using both mono-iodoacetate (MIA) injection into the rat joint cavity and formation of full-thickness defects of rat articular cartilage in vivo, and our results suggested the utility of CCN2 in the regeneration of articular cartilage. This chapter entails helpful tips to apply these two animal models for the evaluation of cartilage-regenerative effects of CCN2 or its derivatives.