A Sorghum propinquum BAC library, suitable for cloning genes associated with loss-of-function mutations during crop domestication
A Sorghum propinquum BAC library, suitable for cloning genes associated with loss-of-function mutations during crop domestication
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DOI：
10.1023/a:1009642201139
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发表时间：
1999-12-01
影响因子：
3.1
通讯作者：
Paterson, AH
中科院分区：
文献类型：
--
作者：
Lin, YR;Zhu, LH;Paterson, AH
构建了一个大型插入高粱 BAC 文库，用于分析高粱基因组的物理组织，并促进与粮食作物驯化早期阶段相关的高粱基因和 QTL 的定位克隆。该文库是使用一个或两个大小选择循环产生的高分子量 DNA 通过 12 种不同的连接建立的。该文库由 38 016 个 BAC 克隆组成，估计平均插入片段大小为 126 kb，覆盖范围为 6.6 个基因组当量。 S. propinquum 的 6.6 基因组等效 BAC 文库提供了 99.7% 的概率找到一种或多种含有感兴趣基因的 BAC。使用 20 个作图 DNA 探针（10 个同源探针和 10 个异源探针）对文库进行筛选，鉴定出 121 个阳性克隆，每个基因座 6.05 个或每个探针 6.37 个。
A large insert Sorghum propinquum BAC library has been constructed to analyze the physical organization of the sorghum genome and to facilitate positional cloning of sorghum genes and QTLs associated with the early stages of grain crop domestication. This library was established from 12 different ligations using high-molecular-weight DNA generated from either one cycle or two cycles of size selection. This library consists of 38 016 BAC clones with an estimated average insert size of 126 kb and coverage of 6.6 genome equivalents. The 6.6 genome-equivalent BAC library of S. propinquum provides a 99.7% probability of finding one or more BACs that contain genes of interest. Twenty mapped DNA probes, ten homologous and ten heterologous, were used to screen the library, and 121 positive clones were identified, 6.05 per locus or 6.37 per probe.