Examination of tissue oxygen saturation (StO2 ) changes associated with vascular pedicle occlusion in a rat Island flap model using near-Infrared spectroscopy.
Examination of tissue oxygen saturation (StO2 ) changes associated with vascular pedicle occlusion in a rat Island flap model using near-Infrared spectroscopy.
复制标题
使用近红外光谱检查大鼠岛状皮瓣模型中与血管蒂闭塞相关的组织氧饱和度 (StO2) 变化。
DOI：
10.1002/micr.22385
复制
发表时间：
2015
期刊：
影响因子：
2.1
通讯作者：
Harii K.
中科院分区：
文献类型：
--
作者：
Kagaya Y;Ohura N;Kurita M;Takushima A;Harii K.
目的本研究的目的是进行连续的StO 2监测大鼠岛状皮瓣在带蒂血管闭塞过程中，近红外光谱（NIRS），以收集实验数据，StO 2皮瓣监测在优化的条件下。将3 × 3cm 2的上腹部岛状皮瓣提升至两侧。将动物随机分配到两组;动脉闭塞组（n= 10）和静脉闭塞组（n= 10）。结果动脉阻断组和静脉阻断组的基础血氧饱和度分别为78.4% ± 3.2%和78.5% ± 5.8%，差异无显著性（P> 0.05）。动脉闭塞后StO 2值立即降低，而静脉闭塞后最初观察到暂时性StO 2升高，随后StO 2降低。血管夹闭后30 min，动脉阻断后StO 2下降27.3% ± 7.1%，静脉阻断后下降28.4% ± 19.1%（P> 0.05）。动脉开放后30 min，StO 2较术前下降0.4% ± 5.8%（P> 0.05），静脉开放后30 min，StO 2较术前下降18.9% ± 11.3%（P< 0.01）。需要进一步的调查，以获得明确的证据与预测程度的皮瓣的活力，并确定实际使用这项技术。© 2015威利期刊公司.显微外科35：393-398，2015年。
PurposeThe purpose of this study was to perform continuous StO2monitoring of rat island flaps during pedicle vessel occlusion using near‐infrared spectroscopy (NIRS) in order to collect experimental data for StO2flap monitoring under optimized conditions.Materials and methodsTwenty rats were used in this study. The 3 × 3 cm2epigastric skin island flaps were elevated on either side. The animals were randomly assigned to two groups; an arterial occlusion group (n= 10) and a venous occlusion group (n= 10). The StO2values of the flaps were observed for over 30 min for the pedicle artery or venous occlusion, followed by an additional 30 min release.ResultsThe baseline StO2value was 78.4% ± 3.2% in the arterial occlusion group, compared to 78.5% ± 5.8% in the venous occlusion group, with no significant differences (P> 0.05). The StO2values decreased immediately after arterial occlusion, whereas a temporal StO2increase was initially observed after venous occlusion, followed by a StO2decrease. The StO2values decreased 27.3% ± 7.1% after arterial occlusion and 28.4% ± 19.1% after venous occlusion at 30 min after pedicle vessel clamping (P> 0.05). The StO2values were 0.4% ± 5.8% lower than baseline 30 min after arterial release (P> 0.05), while 18.9% ± 11.3% lower than baseline 30 min after venous release (P< 0.01).ConclusionNIRS can be used to indicate StO2changes in flaps with the pedicle vessel occlusion and differentiate between pedicle artery and vein occlusion. Further investigations are needed to obtain definitive evidence associated with predicting the degree of flap viability and determine the practical use of this technique. © 2015 Wiley Periodicals, Inc. Microsurgery 35:393–398, 2015.