EphB3 receptor and ligand expression in the adult rat brain
EphB3 receptor and ligand expression in the adult rat brain
复制标题
DOI：
10.1007/s10735-006-9067-0
复制
发表时间：
2006-11-01
影响因子：
3.2
通讯作者：
Miranda, Jorge D.
中科院分区：
文献类型：
--
作者：
Willson, Christopher A.;Foster, Roy D.;Miranda, Jorge D.
Eph受体和配体是在发育过程中控制轴突导向的两个蛋白质家族。它们的表达最初被认为是发育调节的，但最近的工作表明，几种EphA受体在出生后表达。EphB 3受体在胚胎发育期间在中枢神经系统的多个区域中表达，但其在成人脑中的潜在表达和功能作用尚不清楚。我们使用原位杂交，免疫组织化学和受体亲和探针原位染色，研究EphB 3受体mRNA，蛋白质和配体（ephrin-B）的表达，分别在成年大鼠脑。我们的研究结果表明，EphB 3受体的mRNA和蛋白质组成性表达的离散区域的成年大鼠大脑，包括小脑，中缝苍白，海马，内嗅皮质，运动和感觉皮质。EphB 3受体的空间分布共定位于具有高水平EphB 3受体结合配体的脑区域。其表达模式表明，EphB 3可能在发育后期的成熟神经元连接的维持或突触连接的重排中发挥作用。
Eph receptors and ligands are two families of proteins that control axonal guidance during development. Their expression was originally thought to be developmentally regulated but recent work has shown that several EphA receptors are expressed postnatally. The EphB3 receptors are expressed during embryonic development in multiple regions of the central nervous system but their potential expression and functional role in the adult brain is unknown. We used in situ hybridization, immunohistochemistry, and receptor affinity probe in situ staining to investigate EphB3 receptors mRNA, protein, and ligand (ephrin-B) expression, respectively, in the adult rat brain. Our results indicate that EphB3 receptor mRNA and protein are constitutively expressed in discrete regions of the adult rat brain including the cerebellum, raphe pallidus, hippocampus, entorhinal cortex, and both motor and sensory cortices. The spatial profile of EphB3 receptors was co-localized to regions of the brain that had a high level of EphB3 receptor binding ligands. Its expression pattern suggests that EphB3 may play a role in the maintenance of mature neuronal connections or re-arrangement of synaptic connections during late stages of development.