Hematopoietic Cytokines Can Instruct Lineage Choice
Hematopoietic Cytokines Can Instruct Lineage Choice
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DOI：
10.1126/science.1171461
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发表时间：
2009-07-10
期刊：
影响因子：
56.9
通讯作者：
Schroeder, Timm
中科院分区：
文献类型：
--
作者：
Rieger, Michael A.;Hoppe, Philipp S.;Schroeder, Timm
造血过程中血液系统的不断再生需要严格控制造血祖细胞（HPC）的谱系决定。由于技术限制，以前无法连续分析单个HPC的分化。因此，细胞外源性细胞因子是否可以指导HPC谱系选择或仅允许已经谱系限制的细胞存活存在争议。在这里，我们使用生物成像方法，允许连续长期观察个别分化的小鼠HPC。我们证明生理性细胞因子巨噬细胞集落刺激因子和粒细胞集落刺激因子可以指导造血谱系的选择。
The constant regeneration of the blood system during hematopoiesis requires tightly controlled lineage decisions of hematopoietic progenitor cells (HPCs). Because of technical limitations, differentiation of individual HPCs could not previously be analyzed continuously. It was therefore disputed whether cell-extrinsic cytokines can instruct HPC lineage choice or only allow survival of cells that are already lineage-restricted. Here, we used bioimaging approaches that allow the continuous long-term observation of individual differentiating mouse HPCs. We demonstrate that the physiological cytokines, macrophage colony-stimulating factor and granulocyte colony-stimulating factor, can instruct hematopoietic lineage choice.