Adaptation of Saccharomyces cerevisiae to high hydrostatic pressure causing growth inhibition
Adaptation of Saccharomyces cerevisiae to high hydrostatic pressure causing growth inhibition
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DOI：
10.1016/j.febslet.2005.03.100
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发表时间：
2005-05-23
期刊：
影响因子：
3.5
通讯作者：
Kitagawa, E
中科院分区：
文献类型：
--
作者：
Iwahashi, H;Odani, M;Kitagawa, E
全基因组的酿酒酵母在静水压力下生长的mRNA表达谱进行了表征。我们选择在25摄氏度下30 MPa的静水压力，因为酵母细胞能够在这些条件下生长，而减压后细胞大小和复杂性增加。压力诱导基因的功能特征表明，参与蛋白质代谢和膜代谢的基因被诱导。对30 MPa的反应与在致死条件下观察到的反应显著不同，因为蛋白质降解在30 MPa压力下不被激活。强诱导基因：那些有助于膜代谢的基因，也被去污剂、油和膜稳定剂诱导。(c)2005年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
Genome-wide mRNA expression profiles of Saccharomyces cerevisiae growing under hydrostatic pressure were characterized. We selected a hydrostatic pressure of 30 MPa at 25 degrees C because yeast cells were able to grow under these conditions, while cell size and complexity were increased after decompression. Functional characterization of pressure-induced genes suggests that genes involved in protein metabolism and membrane metabolism were induced. The response to 30 MPa was significantly different from that observed under lethal conditions because protein degradation was not activated under 30 MPa pressure. Strongly induced genes those that contribute to membrane metabolism and which are also induced by detergents, oils, and membrane stabilizers. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.