The transcriptional program of sporulation in budding yeast
The transcriptional program of sporulation in budding yeast
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DOI：
10.1126/science.282.5389.699
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发表时间：
1998-10-23
期刊：
影响因子：
56.9
通讯作者：
Herskowitz, I
中科院分区：
文献类型：
--
作者：
Chu, S;DeRisi, J;Herskowitz, I
发芽酵母的二倍体细胞通过孢子形成的发展程序产生单倍体细胞，其中包括减数分裂和孢子形态发生。几乎所有酵母基因的DNA微阵列都用于测定孢子形成过程中基因表达的变化。观察到至少七个不同的诱导时间模式。转录因子NDT80对于在减数分裂预言结束时诱导大量基因，已知或建议负责时间调节的共识序列的大量基因至关重要，这完全可以从共同表达基因的序列的分析中鉴定出来。时间表达模式为数百个先前未表征的基因的潜在功能提供了线索，其中一些基因具有在配子发生过程中可能起作用的脊椎动物同源物。
Diploid cells of budding yeast produce haploid cells through the developmental program of sporulation, which consists of meiosis and spore morphogenesis. DNA microarrays containing nearly every yeast gene were used to assay changes in gene expression during sporulation. At Least seven distinct temporal patterns of induction were observed. The transcription factor Ndt80 appeared to be important for induction of a Large group of genes at the end of meiotic prophase, Consensus sequences known or proposed to be responsible for temporal regulation could be identified solely from analysis of sequences of coordinately expressed genes. The temporal expression pattern provided clues to potential functions of hundreds of previously uncharacterized genes, some of which have vertebrate homologs that may function during gametogenesis.