NMR of Insensitive Nuclei Enhanced by Dynamic Nuclear Polarization
NMR of Insensitive Nuclei Enhanced by Dynamic Nuclear Polarization
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DOI：
10.2533/chimia.2011.260
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发表时间：
2011-01-01
期刊：
影响因子：
1.2
通讯作者：
Bodenhausen, Geoffrey
中科院分区：
文献类型：
--
作者：
Mieville, Pascal;Jannin, Sami;Bodenhausen, Geoffrey
尽管它提供了强大的光谱信息，但核磁共振（NMR）光谱缺乏灵敏度，特别是在处理质子以外的原子核时。尽管NMR在处理有机分子的大量质子时可以以直接的方式应用，但是解决低浓度的生物分子和/或周期表中不提供与质子一样强的信号的许多其他核是非常具有挑战性的。动态核极化（DNP）是一种重要的技术，它提供了一种方法，以显着提高信号强度的NMR。它包括将顺磁中心的非常高的电子自旋极化（通常在低温下）转移到周围的核自旋，并进行适当的微波照射。DNP可以导致核自旋极化的增强高达四个数量级。我们在这篇文章中介绍了DNP的一些基本概念，描述了DNP装置在EPFL，并说明了兴趣的化学应用的技术，通过报告最近的测量（89）Y的络合动力学DOTAM配体。
Despite the powerful spectroscopic information it provides, Nuclear Magnetic Resonance (NMR) spectroscopy suffers from a lack of sensitivity, especially when dealing with nuclei other than protons. Even though NMR can be applied in a straightforward manner when dealing with abundant protons of organic molecules, it is very challenging to address biomolecules in low concentration and/or many other nuclei of the periodic table that do not provide as intense signals as protons. Dynamic Nuclear Polarization (DNP) is an important technique that provides a way to dramatically increase signal intensities in NMR. It consists in transferring the very high electron spin polarization of paramagnetic centers (usually at low temperature) to the surrounding nuclear spins with appropriate microwave irradiation. DNP can lead to an enhancement of the nuclear spin polarization by up to four orders of magnitude. We present in this article some basic concepts of DNP, describe the DNP apparatus at EPFL, and illustrate the interest of the technique for chemical applications by reporting recent measurements of the kinetics of complexation of (89)Y by the DOTAM ligand.