Engineered transmembrane pores.
Engineered transmembrane pores.
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DOI：
10.1016/j.cbpa.2016.08.005
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发表时间：
2016-10
影响因子：
7.8
通讯作者：
Bayley, Hagan
中科院分区：
文献类型：
--
作者：
Ayub, Mariam;Bayley, Hagan
今天，数百名研究人员正在研究纳米孔，对基础科学和生物技术都产生了影响。基于我们以亚纳米精度设计它们的能力，蛋白质仍然是纳米孔最通用的来源。最近的工作旨在构建和发现新的孔隙，包括非自然氨基酸诱变和选择技术的应用。现在，通过螺旋型孔隙以及更为人所知的β桶的发育，增加了结构的多样性。新的发展还包括截断孔，通过脂质重排穿透双分子层，混合孔，完全消除双分子层。孔二聚体，跨越两个脂质双层，已经构建和基于DNA纳米结构的孔越来越重要。虽然纳米孔DNA测序受到了热烈的关注，但蛋白质孔具有更广泛的潜在应用，需要在未来几年继续进行工程努力的规范。
Today, hundreds of researchers are working on nanopores, making an impact in both basic science and biotechnology. Proteins remain the most versatile sources of nanopores, based on our ability to engineer them with sub-nanometer precision. Recent work aimed at the construction and discovery of novel pores has included unnatural amino acid mutagenesis and the application of selection techniques. The diversity of structures has now been increased through the development of helix-based pores as well as the better-known β barrels. New developments also include truncated pores, which pierce bilayers through lipid rearrangement, and hybrid pores, which do away with bilayers altogether. Pore dimers, which span two lipid bilayers, have been constructed and pores based on DNA nanostructures are gaining in importance. While nanopore DNA sequencing has received enthusiastic attention, protein pores have a wider range of potential applications, requiring specifications that will require engineering efforts to continue for years to come.
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影响因子：
4.8
作者：
Hammerstein, Anne F.;Jayasinghe, Lakmal;Bayley, Hagan
通讯作者：
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影响因子：
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作者：
CHANG, CY;NIBLACK, B;BAYLEY, H
通讯作者：
BAYLEY, H