Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.
Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.
复制标题
人类 DNA 聚合酶 δ 催化亚基 cDNA 的分子克隆。
DOI：
10.1093/nar/20.4.735
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发表时间：
1992
影响因子：
14.9
通讯作者：
Lee,MY
中科院分区：
文献类型：
--
作者：
Yang,CL;Chang,LS;Zhang,P;Hao,H;Zhu,L;Toomey,NL;Lee,MY
The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol δ catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol δ is closely related to yeast pol (EQN) and the herpes virus DNA polymerases. The levels of polδ 8 message were found to be induced concomitantly with DNA pol δ activity and DNA synthesis in serum restlmulated proliferating IMR90 cultured cells. The human pol δ8 gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.
