Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability
Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability
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DOI：
10.1016/s1389-1723(99)80049-8
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发表时间：
1999-03-01
影响因子：
2.8
通讯作者：
Urano, K
中科院分区：
文献类型：
--
作者：
Hu, HY;Fujie, K;Urano, K
建立了一种新的活性污泥中微生物醌类化合物的分析方法。优化了用甲醇-氯仿混合物和己烷从活性污泥样品中提取醌类的操作条件。使用Sep-Pak（R）Plus Silica（一种填充有硅胶的小柱）的固相萃取法代替常规方法中使用的柱色谱法和薄层柱色谱法用于醌的纯化和分离。分析操作简单，分析时间比传统方法缩短一半或三分之二。方法的回收率由常规方法的30-60%提高到90-101%，说明改进方法分析结果的可靠性明显高于常规方法。对于相同的活性污泥样品，使用改进的方法获得的醌含量值是使用常规方法获得的醌含量值的两倍高，但是表示为每种醌类型的摩尔分数的醌曲线对于每种方法是相同的。
A novel analytical method for analysis of microbial quinones in activated sludge sample-was developed with improved reliability compared to the conventional method. The operating conditions for the extraction of quinones from activated sludge sample with a methanol-chloroform mixture and hexane were optimized. A solid phase extraction method using the Sep-Pak(R) Plus Silica, a small column packed with silica gel, was employed for the purification and separation of quinones instead of column chromatography and thin layer column chromatography used in the conventional method. The analytical operation was simplified and the analytical time was shortened by a half or two-thirds of that required for the conventional method. The recoveries of quinones were markedly increased from 30-60% with the conventional method to as high as 90-101% with the improved method, demonstrating that the reliability of the analytical results of the improved method is significantly higher than that of the conventional method. The quinone content value obtained using the improved method was twice as high as that obtained using the conventional method for the same activated sludge sample, but the quinone profiles expressed as the mole fraction of each quinone type were the same for each method.