MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community
MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community
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DOI：
10.1007/978-1-4939-6361-4_18
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Britton, Robert A.
中科院分区：
文献类型：
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作者：
Auchtung, Jennifer M.;Robinson, Catherine D.;Britton, Robert A.
共生微生物群在艰难梭菌感染的动态过程中起着重要作用。在本章中，我们描述了微型生物反应器阵列(MBRAs)，这是我们开发的一种体外培养系统，允许在存在复杂粪便微生物群落的情况下对艰难梭菌的生理学进行分析。MBRA内的生物反应器体积很小，可以同时运行数十个反应器，因此可以在有限的时间和成本下测试几个不同的变量。当与艰难梭菌感染动物模型的实验相结合时，MBRAS可以为艰难梭菌的生理学和发病机制提供重要的见解。
The commensal microbiome plays an important role in the dynamics of Clostridium difficile infection. In this chapter, we describe minibioreactor arrays (MBRAs), an in vitro cultivation system that we developed that allows for C. difficile physiology to be assayed in the presence of complex fecal microbial communities. The small size of the bioreactors within the MBRAs allows for dozens of reactors to be run simultaneously and therefore several different variables can be tested with limited time and cost. When coupled with experiments in animal models of C. difficile infection, MBRAs can provide important insights into C. difficile physiology and pathogenesis.