Efficient selection of 3'-terminal exons from vertebrate DNA.
Efficient selection of 3'-terminal exons from vertebrate DNA.
复制标题
从脊椎动物 DNA 中有效选择 3 末端外显子。
DOI：
10.1093/nar/21.22.5198
复制
发表时间：
1993
影响因子：
14.9
通讯作者：
Berget,SM
中科院分区：
文献类型：
--
作者：
Krizman,DB;Berget,SM
基因组DNA中表达序列的鉴定是表征复杂基因组的障碍。我们开发了一个外显子捕获方案，提供了脊椎动物3′-末端外显子的正选择。通过RT/PCR扩增获得捕获的外显子序列的拷贝。该技术检测有效的终端外显子没有干扰部分外显子或非特异性序列，包括简单的人类重复序列。应用于随机人cosmic产生一个独特的捕获的终端外显子平均每个粘粒。由于脊椎动物末端外显子的平均长度为600-700个核苷酸，因此该技术提供了足够长度的转录序列，以帮助进一步的作图工作。
Identification of expressed sequences within genomic DNA is a hurdle in the characterization of complex genomes. We developed an exon trapping scheme that provides a positive selection for vertebrate 3′-terminal exons. A copyof the trapped exon sequence is obtained by RT/PCR amplification. The technique detects valid terminal exons without interference from partial exons or non-specific sequences, including simple human repeated sequences. Application to random human cosmids yielded one unique trapped terminal exon per cosmid on average. Because vertebrate terminal exons average 600–700 nucleotides in length, the technique provides transcribed sequences of sufficient length to assist further mapping efforts.