Use of a partial-filling technique in affinity capillary electrophoresis for determining binding constants of ligands to receptors.
Use of a partial-filling technique in affinity capillary electrophoresis for determining binding constants of ligands to receptors.
复制标题
在亲和毛细管电泳中使用部分填充技术来确定配体与受体的结合常数。
DOI：
10.1016/s0021-9673(99)00278-2
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Gomez,FA
中科院分区：
文献类型：
--
作者：
Heintz,J;Hernandez,M;Gomez,FA
这项工作评估了亲和毛细管电泳（ACE）中的部分填充技术的概念，使用两个模型系统：万古霉素从东方链霉菌和碳酸酐酶B（CA B，EC 4.2.1.1）。在该技术中，首先用配体部分填充毛细管，然后用受体和非相互作用标准品的样品填充毛细管并进行电泳。分析受体相对于非相互作用标准品的迁移率比M的变化，作为配体浓度的函数，得到结合常数的值。这些值与使用其他绑定和ACE技术估计的值一致。数据表明，这种方法的定量潜力。
This work evaluates the concept of a partial-filling technique in affinity capillary electrophoresis (ACE) using two model systems: vancomycin from Streptomyces orientalis and carbonic anhydrase B (CAB, EC 4.2.1.1). In this technique the capillary is first partially-filled with ligand followed by a sample of receptor and non-interacting standard and electrophoresed. Analysis of the change in the mobility ratio, M, of the receptor, relative to the non-interacting standard, as a function of the concentration of the ligand, yields a value for the binding constant. These values agree well with those estimated using other binding and ACE techniques. Data demonstrating the quantitative potential of this method is presented.