Organization of the fibronectin gene provides evidence for exon shuffling during evolution.
Organization of the fibronectin gene provides evidence for exon shuffling during evolution.
复制标题
纤连蛋白基因的组织为进化过程中的外显子改组提供了证据。
DOI：
10.1002/j.1460-2075.1987.tb02545.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Hynes,RO
中科院分区：
文献类型：
--
作者：
Patel,RS;Odermatt,E;Schwarzbauer,JE;Hynes,RO
我们报告的组织的两端的大鼠纤连蛋白基因编码的I型和II型重复单位的蛋白质。我们发现，这些模块化的结构单位是由一个单独的外显子编码。已知同源I型和II型重复序列存在于组织纤溶酶原激活物、因子XII和牛精浆蛋白中。这些序列和纤连蛋白和组织纤溶酶原激活剂基因的外显子结构的比较表明，编码I型和II型重复的外显子在进化过程中重排。我们还报告了纤连蛋白基因的5′端和3′端的分析，包括启动子区和编码纤连蛋白前体序列的外显子，我们表明该基因是从一个单一的起始位点转录到一个单一的多聚腺苷酸化位点。这些数据提供了与基因的转录调控、初级转录物的选择性剪接和初级翻译产物的结构有关的信息。
We report the organization of the two ends of the rat fibronectin gene which encode the type I and II repeating units of the protein. We show that each of these modular structural units is encoded by a separate exon. Homologous type I and II repeats are known to occur in tissue plasminogen activator, factor XII and a bovine seminal plasma protein. Comparison of these sequences and the exon structures of the fibronectin and tissue plasminogen activator genes indicates that exons encoding type I and type II repeats have reassorted during evolution. We also report analyses of the extreme 5′ and 3′ ends of the fibronectin gene including the promoter region and the exon encoding the prepro sequence of fibronectin and we show that the gene is transcribed from a single initiation site to a single polyadenylation site. These data provide information pertinent to the transcriptional regulation of the gene, the alternative splicing of the primary transcript and the structure of the primary translation product.