Invasion of human coronary artery endothelial cells by Streptococcus mutans OMZ175.
Invasion of human coronary artery endothelial cells by Streptococcus mutans OMZ175.
复制标题
DOI：
10.1111/j.1399-302x.2008.00487.x
复制
发表时间：
2009-04
影响因子：
--
通讯作者：
Burne RA
中科院分区：
文献类型：
--
作者：
Abranches J;Zeng L;Bélanger M;Rodrigues PH;Simpson-Haidaris PJ;Akin D;Dunn WA Jr;Progulske-Fox A;Burne RA
口腔细菌传播到血液中与饮食、口腔卫生和牙科手术有关;包括拔牙、牙髓治疗和牙周手术。最近，研究确定变形链球菌，龋齿的主要病原体，作为最普遍的细菌物种中发现的临床样本谁经历了心脏瓣膜和动脉粥样硬化斑块手术的患者。采用抗生素保护试验，对14株S.变形杆菌侵袭原代人冠状动脉内皮细胞（HCAEC）。血清型e株B14和血清型f株OMZ 175。变形菌能够有效地侵入HCAEC。在供试菌株中，血清型f S.变形杆菌OMZ 175是最具侵袭性的，而血清型c S.牙菌斑中最普遍的血清型-变形菌，不具有侵袭性。基于其高侵袭率，我们进一步研究了血清型f OMZ 175的侵袭特性。利用透射电子显微镜和抗生素保护试验，我们证明了S。变形杆菌OMZ 175能够附着于HCAEC表面，进入细胞并在HCAEC中存活至少29小时。我们的研究结果强调了S.在某些心血管疾病的发病机制中的变异。
Dissemination of oral bacteria into the bloodstream has been associated with eating, oral hygiene, and dental procedures; including tooth extraction, endodontic treatment, and periodontal surgery. Recently, studies identified Streptococcus mutans, the primary etiological agent of dental caries, as the most prevalent bacterial species found in clinical samples from patients who underwent heart valve and atheromatous plaque surgery. By using antibiotic protection assays, we tested the capacity of 14 strains of S. mutans to invade primary human coronary artery endothelial cells (HCAEC). Serotype e strain B14 and serotype f strain OMZ175 of S. mutans were able to efficiently invade HCAEC. Among the tested strains, serotype f S. mutans OMZ175 was the most invasive, whereas strains of serotype c S. mutans, the most prevalent serotype in dental plaque, were not invasive. Based on its high invasion rate, we further investigated the invasive properties of serotype f OMZ175. Using transmission electron microscopy and antibiotic protection assays we demonstrate that S. mutans OMZ175 is capable of attaching to the HCAEC surface, entering the cells and surviving in HCAEC for at least 29 h. Our findings highlight a potential role for S. mutans in the pathogenesis of certain cardiovascular diseases.