IMPROVED PRESERVATION OF THE FORM AND CONTENTS OF WALL VESICLES AND THE GOLGI-APPARATUS IN FREEZE SUBSTITUTED HYPHAE OF SAPROLEGNIA
IMPROVED PRESERVATION OF THE FORM AND CONTENTS OF WALL VESICLES AND THE GOLGI-APPARATUS IN FREEZE SUBSTITUTED HYPHAE OF SAPROLEGNIA
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DOI：
10.1007/bf01276331
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
OTTENSMEYER, FP
中科院分区：
文献类型：
--
作者：
HEATH, IB;RETHORET, K;OTTENSMEYER, FP
分泌囊泡参与细胞壁合成（壁囊泡）和高尔基体已在常规固定和冷冻替代的卵菌真菌水霉菌丝进行了比较。壁囊泡冷冻替代各种流体范围从球形到管状，并含有强烈染色，富磷基质。相反，不同的常规固定导致大多数管状囊泡中的人为收缩和其强烈染色内容物的损失。这些数据被解释为显示囊内骨骼系统的存在，与细胞调节，以确定囊泡形态和囊内合成一个假设的磷酸化糖脂细胞壁前体。虽然冷冻置换提供了上级保存壁囊泡形态，它没有表现出任何优先关联壁囊泡和微管，从而表明微管只是间接参与壁囊泡运输。冷冻置换是上级的常规固定分析的高尔基体，因为它独特地揭示了一个特定的单池在每个高尔基体的分化和整个内膜系统的膜厚度更大的差异。
Secretory vesicles involved in cell wall synthesis (wall vesicles) and the Golgi apparatus have been compared in conventionally fixed and freeze substituted hyphae of the oomycete fungus Saprolegnia ferax. Wall vesicles freeze substituted in various fluids range from spherical to tubular and contain an intensely staining, phosphorus rich matrix. In contrast diverse conventional fixations cause artefactual constrictions in most tubular vesicles and loss of their intensely staining contents. These data are interpreted to show the existence of an intravesicular skeletal system, with cellular regulation, to determine vesicle morphology and intravesicular synthesis of a hypothetical phosphorylated glycolipid cell wall precursor. Whilst freeze substitution gives superior preservation of wall vesicle morphology, it does not demonstrate any preferential association between wall vesicles and microtubules thus suggesting that microtubules are only indirectly involved in wall vesicle transport. Freeze substitution is superior to conventional fixation for analysis of the Golgi apparatus because it uniquely reveals both differentiation of a specific single cisterna in each Golgi body and greater differences in membrane thickness throughout the endomembrane system.