Betulinic acid induces apoptosis and ultrastructural changes in MDA-MB-231 breast cancer cells
Betulinic acid induces apoptosis and ultrastructural changes in MDA-MB-231 breast cancer cells
复制标题
桦木酸诱导 MDA-MB-231 乳腺癌细胞凋亡和超微结构变化
DOI：
10.1080/01913123.2017.1383548
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发表时间：
2018
影响因子：
1
通讯作者：
Jingwei Zhang
中科院分区：
文献类型：
--
作者：
Yang Gao;Qing Ma;Yanbing Ma;L. Ding;Xiaolong Xu;Defei Wei;Lei Wei;Jingwei Zhang
摘要本研究旨在探讨白桦酸(BA)对三阴性乳腺癌MDA-MB-231细胞的作用及其超微结构的变化。BA诱导MDA-MB-231细胞凋亡所需的浓度已有报道。本研究采用细胞计数试剂盒-8增殖实验检测细胞存活率和凋亡率。Western blotting检测细胞内Bcl2蛋白表达水平。显微镜下观察细胞形态和细胞密度的变化。电子显微镜下可见固缩的细胞核和空泡。综上所述，我们的结果揭示了BA损伤MDA-MB-231细胞超微结构的形态学机制。
ABSTRACT The aim of this study is to investigate the effects of betulinic acid (BA) on triple-negative breast cancer MDA-MB-231 cells and observe the ultrastructural changes. The concentration of BA required to induce apoptosis in MDA-MB-231 cells has been previously reported. In this study, a cell counting kit-8 proliferation assay was used to measure cell viability and the apoptosis rate. Western blotting was performed to observe the protein expression levels of Bcl-2. Cell morphology and changes in cell density were observed by microscopy. Electron microscopy revealed pyknotic nuclei as well as vacuoles. Collectively, our results showed the morphological mechanisms by which BA impairs the ultrastructure of MDA-MB-231 cells.