Genetic marking of sex using a W chromosome-linked transgene
Genetic marking of sex using a W chromosome-linked transgene
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DOI：
10.1016/j.ibmb.2013.08.009
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发表时间：
2013-12-01
影响因子：
3.8
通讯作者：
Xia, Qingyou
中科院分区：
文献类型：
--
作者：
Ma, Sanyuan;Wang, Xiaogang;Xia, Qingyou
许多属于鳞翅目的物种是农业和树木栽培中的主要害虫。昆虫不育技术（SIT）是一种生态友好、高效的基因靶向害虫防治方法。在许多情况下，最好在昆虫不育技术计划中仅释放不育雄性，而有效的性别鉴定策略对于成功大规模实施昆虫不育技术至关重要。在本研究中，我们建立了 160 个转基因蚕（Bombyx mori）品系，以测试使用 W 染色体连锁转基因进行遗传性别鉴定的可能性，这被认为是鳞翅目物种的最佳性别鉴定策略。获得了具有雌性特异性报告基因表达模式的转基因系。 W连锁转基因的表达水平与常染色体插入相当，并且连续17代保持稳定。分子表征显示该系在 W 染色体上含有报告基因的单个拷贝，并且整合位点是重叠群 W-BAC-522N19-C9 中的 TTAG。这里展示的使用 W 染色体连锁转基因的可行性以及讨论的可能的改进将为其他鳞翅目害虫提供有价值的信息。本研究建立的新型W染色体连锁转基因系将成为家蚕基础研究的重要资源。 (C) 2013 Elsevier Ltd. 保留所有权利。
Many species belonging to the order Lepidoptera are major pests in agriculture and arboriculture. The sterile insect technique (SIT) is an eco-friendly and highly efficient genetically targeted pest management approach. In many cases, it is preferable to release only sterile males in an SIT program, and efficient sexing strategies are crucial to the successful large-scale implementation of SIT. In the present study, we established 160 transgenic silkworm (Bombyx mori) lines to test the possibility of genetic sexing using a W chromosome-linked transgene, which is thought to be the best sexing strategy for lepidopteran species. One transgenic line with a female-specific expression pattern of reporter gene was obtained. The expression level of the W-linked transgene was comparable with autosomal insertions and was stable for 17 continuous generations. Molecular characterization showed this line contained a single copy of the reporter gene on the W chromosome, and the integration site was TTAG in contig W-BAC-522N19-C9. The feasibility of using a W chromosome-linked transgene demonstrated here and the possible improvements discussed will provide valuable information for other lepidopteran pests. The novel W chromosome-linked transgenic line established in this study will serve as an important resource for fundamental research with the silkworm B. mori. (C) 2013 Elsevier Ltd. All rights reserved.