TAT-peroxiredoxin 2 Fusion Protein Supplementation Improves Sperm Motility and DNA Integrity in Sperm Samples from Asthenozoospermic Men
TAT-peroxiredoxin 2 Fusion Protein Supplementation Improves Sperm Motility and DNA Integrity in Sperm Samples from Asthenozoospermic Men
复制标题
TAT-过氧化还原蛋白 2 融合蛋白补充剂可改善弱精子症男性精子样本中的精子活力和 DNA 完整性。
DOI：
10.1016/j.juro.2015.11.019
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发表时间：
2016-03-01
影响因子：
6.6
通讯作者：
Li, Yan
中科院分区：
文献类型：
--
作者：
Liu, Juan;Zhu, Peng;Li, Yan
目的：我们比较了正常精子和弱精子男性精液样本中过氧化物还蛋白2的水平。还评估了tat -过氧化物还氧蛋白2融合蛋白对精子活力和DNA完整性的潜在影响。材料与方法：取50例正常精子者和50例弱精子者的精液样本。使用商用丙二醛试剂盒测定精液脂质过氧化。TUNEL法检测精子DNA片段。采用Western blot和免疫荧光法检测精浆和精子中过氧化物还氧蛋白2的含量。在精子悬液中添加tat -过氧还蛋白2融合蛋白孵育2和12小时后，评估精子活力、DNA损伤和活性氧水平。结果：在弱精子精子样本中发现明显较高水平的丙二醛和DNA损伤。然而，与正常精子男性相比，精浆和精子中过氧化物还蛋白2的表达明显降低。成功制备了tat -过氧化物还氧蛋白2融合蛋白并将其传递到精子中。有趣的是，在弱精子精子悬浮液中加入tat -过氧化物还氧蛋白2可以有效地防止进行性运动能力下降和DNA损伤增加。结论：本研究表明，在弱动精子症男性的精子悬浮液中添加tat -过氧化物还蛋白2融合蛋白可以通过降低活性氧水平有效改善精子活力和DNA完整性。因此，我们推测过氧化物氧还蛋白2可能在精液中具有重要的抗氧化防御作用，并将为弱精子症的预防和治疗提供新的选择。
Purpose: We compared levels of peroxiredoxin 2 in semen samples from normozoospermic and asthenozoospermic men. The potential effects of TAT-peroxiredoxin 2 fusion protein on sperm motility and DNA integrity were also evaluated.Materials and Methods: Semen samples were obtained from 50 normozoospermic and 50 asthenozoospermic men. Lipid peroxidation of semen was determined using a commercial malondialdehyde kit. Sperm DNA fragmentation was evaluated by TUNEL assay. Western blot and immunofluorescence were performed to detect the amount of peroxiredoxin 2 protein in seminal plasma and spermatozoa. Sperm motility, DNA damage and levels of reactive oxygen species were evaluated after TAT-peroxiredoxin 2 fusion protein supplementation to the sperm suspension for 2 and 12 hours of incubation.Results: In asthenozoospermic semen samples a significantly higher level of malondialdehyde and DNA damage was discovered. However, the expression of peroxiredoxin 2 was significantly lower in seminal plasma and spermatozoa compared with that of normozoospermic men. TAT-peroxiredoxin 2 fusion protein was successfully prepared and delivered to the spermatozoa. Interestingly adding TAT-peroxiredoxin 2 in asthenozoospermic sperm suspension effectively defended against the decrease in progressive motility and the increase in DNA damage.Conclusions: This study shows that supplementation of TAT-peroxiredoxin 2 fusion protein in the sperm suspension from asthenozoospermic men effectively improved sperm motility and DNA integrity by reducing levels of reactive oxygen species. Therefore, we speculate that peroxiredoxin 2 may have an important role as an antioxidant defense in semen and would provide new prevention and therapy alternatives for asthenozoospermia.