Fueling the engine: induction of AMP-activated protein kinase in trout skeletal muscle by swimming
Fueling the engine: induction of AMP-activated protein kinase in trout skeletal muscle by swimming
复制标题
DOI：
10.1242/jeb.099192
复制
发表时间：
2014-05-01
影响因子：
2.8
通讯作者：
Planas, Josep V.
中科院分区：
文献类型：
--
作者：
Magnoni, Leonardo J.;Palstra, Arjan P.;Planas, Josep V.
AMP活化蛋白激酶（AMPK）是一种运动诱导的蛋白激酶，在哺乳动物骨骼肌中起重要的代谢调节作用。尽管运动作为运动肌肉能量利用的调节剂具有生理重要性，但在鱼类中尚未研究过游泳对这种酶的调节。我们发现，持续游泳（40天，0.75体长s（-1））增加AMPK活性在红色和白色鳟鱼骨骼肌（分别为3.9倍和2.2倍），以及AMPK的目标基因的表达参与能量的用途：脂蛋白脂肪酶和柠檬酸合成酶在红色和白色肌肉和CPT 1 β 1 b和PGC-1 α在红色肌肉。此外，电脉冲刺激培养鳟鱼肌管增加AMPK活性和葡萄糖摄取（1.9和1.2倍，分别）在AMPK依赖的方式。这些结果表明AMPK在鱼类骨骼肌游泳代谢适应中可能起重要的介导作用。
AMP-activated protein kinase (AMPK) is well known to be induced by exercise and to mediate important metabolic changes in the skeletal muscle of mammals. Despite the physiological importance of exercise as a modulator of energy use by locomotory muscle, the regulation of this enzyme by swimming has not been investigated in fish. We found that sustained swimming (40 days at 0.75 body lengths s(-1)) increased AMPK activity in red and white trout skeletal muscle (3.9- and 2.2-fold, respectively) as well as the expression of AMPK target genes involved in energy use: lipoprotein lipase and citrate synthase in red and white muscle and CPT1 beta 1b and PGC-1 alpha in red muscle. Furthermore, electrical pulse stimulation of cultured trout myotubes increased AMPK activity and glucose uptake (1.9- and 1.2-fold, respectively) in an AMPK-dependent manner. These results suggest that AMPK may play an important mediatory role in the metabolic adaptation to swimming in fish skeletal muscle.