Sandwich freezing device for rapid freezing of viruses, bacteria, yeast, cultured cells and animal and human tissues in electron microscopy
Sandwich freezing device for rapid freezing of viruses, bacteria, yeast, cultured cells and animal and human tissues in electron microscopy
复制标题
三明治冷冻装置，用于在电子显微镜下快速冷冻病毒、细菌、酵母、培养细胞以及动物和人体组织
DOI：
10.1093/jmicro/dfaa049
复制
发表时间：
2020
期刊：
影响因子：
1.8
通讯作者：
Chibana Hiroji
中科院分区：
文献类型：
--
作者：
Yamaguchi Masashi;Taguchi Masaki;Uematsu Katsuyuki;Takahashi-Nakaguchi Azusa;Sato-Okamoto Michiyo;Chibana Hiroji
我们一直在使用活酵母和细菌的三明治冷冻，然后进行冷冻置换，以观察接近天然的细胞超微结构。最近，人们发现，用戊二醛固定的培养细胞和人体组织的夹心冷冻可以很好地保存细胞和组织的超微结构。然而，这些研究是使用手工三明治冷冻装置进行的，并且仅限于少数实验室。为了将这种方法推广到其他实验室，我们制造并商业化了一种新的三明治冷冻装置。这种新设备价格低廉，便于携带，而且可以消毒。如果组织预先用戊二醛固定，则可用于快速冷冻病毒、细菌、酵母、培养细胞以及动物和人体组织至0.2 mm的深度。该装置的商业可用性将快速冷冻的应用扩展到广泛的生物材料。
We have been using sandwich freezing of living yeast and bacteria followed by freeze-substitution for observing close-to-native ultrastructure of cells. Recently, sandwich freezing of glutaraldehyde-fixed cultured cells and human tissues have been found to give excellent preservation of ultrastructure of cells and tissues. These studies, however, have been conducted using a handmade sandwich freezing device and have been limited in a few laboratories. To spread the use of this method to other laboratories, we fabricated and commercialized a new sandwich freezing device. The new device is inexpensive, portable and sterilizable. It can be used to rapid-freeze viruses, bacteria, yeast, cultured cells and animal and human tissues to a depth of 0.2 mm if tissues are prefixed with glutaraldehyde. The commercial availability of this device will expand application of rapid freezing to wide range of biological materials.