Nanoengineered structures for holding and manipulating liposomes and cells.
Nanoengineered structures for holding and manipulating liposomes and cells.
复制标题
用于容纳和操纵脂质体和细胞的纳米工程结构。
DOI：
10.1021/ac001020m
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发表时间：
2001
影响因子：
7.4
通讯作者：
Zare,RN
中科院分区：
文献类型：
--
作者：
Wilson,CF;Simpson,GJ;Chiu,DT;Strömberg,A;Orwar,O;Rodriguez,N;Zare,RN
We describe the fabrication of nanoengineered holding pipets with concave seating surfaces and fine pressure control. These pipets were shown to exhibit exceptional stability in capturing, transporting, and releasing single cells and liposomes 1−12 μm in diameter, which opens previously inaccessible avenues of research. Three specific examples demonstrated the utility and versatility of this manipulation system. In the first, carboxyrhodamine was selectively incorporated into individual cells by electroporation, after which nearly all the medium (hundreds of microliters) surrounding the docked and tagged cells was rapidly exchanged (in seconds) and the cells were subsequently probed by laser-induced fluorescence (LIF). In the second study, a single liposome containing car-boxyrhodamine was transported to a dye-free solution using a transfer pipet, docked to a holding pipet, and held firmly during physical agitation and interrogation by LIF. In the third study, pairs of liposomes were positioned between two microelectrodes, held in contact, and selectively electrofused and the resulting liposomes undocked intact.
