A PROTEIN-COMPONENT OF DROSOPHILA POLAR GRANULES IS ENCODED BY VASA AND HAS EXTENSIVE SEQUENCE SIMILARITY TO ATP-DEPENDENT HELICASES
A PROTEIN-COMPONENT OF DROSOPHILA POLAR GRANULES IS ENCODED BY VASA AND HAS EXTENSIVE SEQUENCE SIMILARITY TO ATP-DEPENDENT HELICASES
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DOI：
10.1016/0092-8674(88)90216-4
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发表时间：
1988-11-18
期刊：
影响因子：
64.5
通讯作者：
JAN, YN
中科院分区：
文献类型：
--
作者：
HAY, B;JAN, LY;JAN, YN
极细胞的决定簇是生殖细胞的前体，由母体提供，并定位于果蝇卵的后极，极颗粒也是如此。据推测，某些RNA分子与极性颗粒可能是必要的极细胞的决定。使用单克隆抗体（Mab 46 F11）对极性颗粒，我们已经克隆了它们的组成部分之一的基因。这个基因被证明是vasa，这是形成极性颗粒和生殖细胞所必需的。该极性颗粒组分显示与eIF-4A（结合mRNA的翻译起始因子）和其它解旋酶的显著序列相似性。
Determinants of pole cells, which are precursors of the germ line, are provided maternally and are localized to the posterior pole of the Drosophila egg, as are polar granules. It has been hypothesized that certain RNA molecules associated with polar granules may be necessary for pole cell determination. Using a monoclonal antibody (Mab46F11) against polar granules, we have cloned the gene for one of their components. This gene turns out to be vasa, which is required maternally for the formation of polar granules and germ cells. This polar granule component shows significant sequence similarity to elF-4A, a translation initiation factor that binds to mRNA, and to other helicases.