Distinct expression of NK2 homeobox 1 (NKX2-1) and goblet cell hyperplasia in nasal polyps with different endotypes
Distinct expression of NK2 homeobox 1 (NKX2-1) and goblet cell hyperplasia in nasal polyps with different endotypes
复制标题
不同内型鼻息肉中 NK2 同源盒 1 (NKX2-1) 的独特表达和杯状细胞增生。
DOI：
10.1002/alr.21932
复制
发表时间：
2017-07-01
影响因子：
6.4
通讯作者：
Li, Huabin
中科院分区：
文献类型：
--
作者：
Du, Jintao;Ba, Luo;Li, Huabin
背景资料：哮喘患者气道上皮特异性转录因子NK 2同源框1（NKX 2 -1）表达降低与过敏性炎症相关。方法：检测鼻息肉组织中主要细胞因子（IL-4、IL-5、IL-13、IL-17 A等）的表达，并与正常鼻息肉组织进行比较。结果：23%的慢性鼻窦炎（CRS）伴NP（CRSwNP）患者存在IL-5（+）嗜酸性粒细胞，40.7%的NP为关键细胞因子阴性（KCN NP），且嗜酸性粒细胞聚集较少。IL-5（+）NPs中NKX 2 -1的表达显著低于KCN NPs和正常对照组（p < 0.05）。IL-5（+）NPs中粘蛋白5AC（MUC 5AC）和MUC 5 B的表达以及杯状细胞增生显著升高，这与NKX 2 -1表达的降低相关（p < 0.05）。此外，IL-5（+）NPs中“含有ETS转录因子的SAM指向结构域”（SPDEF）显著升高，而叉头盒A2（FoxA 2）的表达显著降低（p < 0.05）。结论：IL-5 + NPs中NKX 2 -1表达下调可能与组织嗜酸性粒细胞增多和杯状细胞增生有关。(C)2017 ARS-AAOA，LLC.
Background: Decreased expression of airway epithelialspecific transcription factor NK2 homeobox 1 (NKX2-1) was associated with allergic inflammation in asthma patients. However, the expression and role of NKX2-1 in nasal polyps (NPs) with different endotypes were undefined yet.Methods: We examined the expression of key cytokines (interleukin [IL]-4 IL-5, IL-13, and IL-17A, etc.) and NKX21 in NPs with different endotypes and control tissues by immunohistochemistry staining, qualitative polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA), andWestern blot analysis.Results: We found 23% of chronic rhinosinusitis (CRS) with NP (CRSwNP) patients had IL-5(+) eosinophilic NPs, 40.7% of NPs were key cytokines negative NPs (KCN NPs) with less eosinophil accumulation. The expression of NKX2-1 in IL-5(+) NPs was significantly lower than KCN NPs and normal controls (p < 0.05). The expression of mucin 5AC (MUC5AC) and MUC5B, as well as goblet cells hyperplasia, were significantly elevated in IL-5(+) NPs, which correlated with the decreased expression of NKX2-1 (p < 0.05). Moreover, "SAMpointed domain containing ETS transcription factor" (SPDEF) was significantly elevated, while expression of Forkhead Box A2 (FoxA2) was significantly decreased in IL-5(+) NPs (p < 0.05). The expression of chemokine (C-C motif) ligand 17 (CCL17) and IL-4 was significantly increased in IL-5+ NPs, which was associated with eosinophil accumulation(p < 0.05).Conclusion: The downregulation of NKX2-1 in IL-5+ NPs may be associated with tissue eosinophilia and goblet cells hyperplasia. (C) 2017 ARS-AAOA, LLC.