First Molecular Characterization of Avian Metapneumovirus (aMPV) in Turkish Broiler Flocks
First Molecular Characterization of Avian Metapneumovirus (aMPV) in Turkish Broiler Flocks
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DOI：
10.1637/11915-061818-resnote.1
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发表时间：
2018-12-01
期刊：
影响因子：
1.4
通讯作者：
Yilmaz, H.
中科院分区：
文献类型：
--
作者：
Bayraktar, E.;Umar, S.;Yilmaz, H.
病毒性呼吸道疾病，包括禽偏肺病毒（aMPV），对家禽业有重大的经济影响。目前还不清楚土耳其肉鸡群中aMPV的频率和基因型多样性。本研究的目的是报告在土耳其肉鸡群中传播的aMPV的第一个分子鉴定和繁殖。从2017年3月至2018年3月期间分布在土耳其不同地理区域的110个肉鸡群中采集气管组织样本和气管拭子。使用通用逆转录酶（RT）PCR确认了aMPV的检测，8个（7.2%）肉鸡养殖场aMPV呈阳性。G基因的序列分析揭示了B亚型病毒的唯一存在。三个田间分离株与2002年以色列分离株紧密聚集，表明这两个国家之间以及通过中东的潜在传播途径。其余5株田间分离株与疫苗株密切相关，尽管土耳其的肉鸡群未常规接种aMPV疫苗。因此，我们推测这五个分离株可能来自附近的接种火鸡农场。此外，与参考疫苗序列相比，存在一些核苷酸取代，表明原始疫苗病毒或疫苗亚群在田间条件下选择的循环和进化延长。这一证据强调需要进一步详细和更系统的方法来评估aMPV的传播和演变，以设计有效的控制策略。
Viral respiratory diseases, including avian metapneumovirus (aMPV), have a significant economic impact on poultry industries. The frequency and genotype diversity of aMPV in Turkish broiler flocks is not known at present. The aim of this study was to report the first molecular identification and phylogeny of aMPV, which is circulating in Turkish broiler flocks. Trachea tissue samples and tracheal swabs were collected from 110 broiler flocks distributed in different geographical regions in Turkey between March 2017 and March 2018. Detection of aMPV was confirmed with the use of universal reverse transcriptase (RT) PCR, and eight (7.2%) broiler farms were positive for aMPV. Sequence analysis of the G gene revealed the exclusive presence of subtype B viruses. Three field isolates clustered closely with a 2002 Israel isolate, indicating a potential transmission route between these two countries and through the Middle East. The remaining five field isolates were closely related to a vaccine strain, even though broiler flocks in Turkey are not routinely vaccinated against aMPV. Therefore, we speculate these five isolates could have originated from nearby vaccinated turkey farms. Additionally, the presence of some nucleotide substitutions compared to the reference vaccine sequence suggests prolonged circulation and evolution of the original vaccine virus or a vaccine subpopulation was selected under field conditions. This evidence emphasizes the need for further detailed and more systemic approaches to evaluate aMPV spread and evolution in order to design effective control strategies.