MSLO, A COMPLEX MOUSE GENE ENCODING MAXI CALCIUM-ACTIVATED POTASSIUM CHANNELS
MSLO, A COMPLEX MOUSE GENE ENCODING MAXI CALCIUM-ACTIVATED POTASSIUM CHANNELS
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DOI：
10.1126/science.7687074
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发表时间：
1993-07-09
期刊：
影响因子：
56.9
通讯作者：
SALKOFF, L
中科院分区：
文献类型：
--
作者：
BUTLER, A;TSUNODA, S;SALKOFF, L
从小鼠脑和骨骼肌中分离出编码钙激活钾通道的基因mSlo的互补DNA（cDNA），进行测序，并在非洲爪蟾卵母细胞中表达。mSlo编码的通道类似于“maxi "或BK（高电导）通道类型;单通道电导为272皮西门子，钾浓度对称。全细胞和单通道电流被阻断的charybdotoxin，伊比利亚毒素，和四乙基铵离子。大量的变体mSlo cDNA被分离，表明几种不同的哺乳动物BK通道类型由单个基因产生。
Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled ''maxi'' or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.