WIDESPREAD OCCURRENCE OF 87 KDA, A MAJOR SPECIFIC SUBSTRATE FOR PROTEIN-KINASE-C
WIDESPREAD OCCURRENCE OF 87 KDA, A MAJOR SPECIFIC SUBSTRATE FOR PROTEIN-KINASE-C
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DOI：
10.1073/pnas.83.9.2822
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发表时间：
1986-05-01
影响因子：
11.1
通讯作者：
GREENGARD, P
中科院分区：
文献类型：
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作者：
ALBERT, KA;WALAAS, SI;GREENGARD, P
一种87 kda的磷酸蛋白，以前被认为是大鼠脑破碎细胞制剂中Ca2+/磷脂/二酰基甘油依赖性蛋白激酶（蛋白激酶C）的主要特异性底物，已经被表征为其种类、组织和亚细胞分布。在猴子、人类、老鼠和牛的大脑以及加利福尼亚鱼雷的电器官中也发现了类似的蛋白质。该蛋白也在多种非神经元大鼠和牛组织中被鉴定出来。与牛组织中的蛋白相比，大鼠蛋白的表观分子质量低4-7 kDa，酸性稍强。各种牛组织的87-kDa蛋白在以下标准上是相同的：每个蛋白都被外源蛋白激酶C磷酸化，具有相当的分子质量，等电聚焦时在4.4-4.9的pH范围内产生多个斑点，与金黄色葡萄球菌V8蛋白酶消化时产生相同的模式，并且被特异性的87-kDa抗血清识别。牛组织中87-kDa蛋白的相对浓度在脑、脾和肺中最高，在睾丸、胰腺、肾上腺、肾脏和肝脏中中等，在心脏和骨骼肌中最低。在大脑中，87-kDa蛋白集中在突触体膜和细胞质中。膜结合蛋白可以用非离子洗涤剂提取，但不能用NaCl提取。这种87-kDa蛋白的种、组织和亚细胞分布与蛋白激酶C相似。
An 87-kDa phosphoprotein, identified previously as a mjaor, specific substrate for Ca2+/phospholipid/diacylglycerol-dependent protein kinase (protein kinase C) in broken cell preparations from rat brain, has been characterized with respect to its species, tissue, and subcellular distribution. A similar protein was present in monkey, human, mouse, and bovine brain and in Torpedo californica electric organ. The protein was also identified in a variety of non-neuronal rat and bovine tissues. The rat protein had an apparent molecular mass 4-7 kDa lower, and was slightly more acidic, than the protein in bovine tissues. The 87-kDa proteins from various bovine tissues were identical by the following criteria: each was phosphorylated by exogenous protein kinase C, was of comparable molecular mass, generated multiple spots within the pH range of 4.4-4.9 upon isoelectric focusing, yielded identical patterns upon digestion with Staphylococcus aureus V8 protease, and was recognized by a specific 87-kDa antiserum. The relative concentrations of the 87-kDa protein in bovine tissues were highest in brain, spleen, and lung, moderate in testis, pancreas, adrenal, kidney, and liver, and lowest in heart and skeletal muscle. In the brain, the 87-kDa protein was concentrated in the synaptosomal membrane and in the cytosol. The membrane-bound protein was extractable with nonionic detergents but not with NaCl. This species, tissue, and subcellular distribution of the 87-kDa protein is similar to that of protein kinase C.