Measurement of DNA Translocation Dynamics in a Solid-State Nanopore at 100 ns Temporal Resolution.
Measurement of DNA Translocation Dynamics in a Solid-State Nanopore at 100 ns Temporal Resolution.
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DOI：
10.1021/acs.nanolett.6b01661
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发表时间：
2016-07-13
期刊：
影响因子：
10.8
通讯作者：
Shepard KL
中科院分区：
文献类型：
--
作者：
Shekar S;Niedzwiecki DJ;Chien CC;Ong P;Fleischer DA;Lin J;Rosenstein JK;Drndić M;Shepard KL
尽管纳米孔有潜力成为单分子系统高带宽研究的平台，但通过纳米孔进行的离子电流测量的时间分辨率受到优化不良的测量电子设备和纳米孔膜中大寄生电容产生的噪声的限制。在这里，我们提出了一种互补金属氧化物半导体 (CMOS) 纳米孔 (CNP) 放大器，能够以前所未有的 10 MHz 带宽进行低噪声记录。当与氮化硅膜中最先进的固态纳米孔集成时，我们在 5 MHz 的测量带宽下实现了 ssDNA 易位的 SNR 大于 10，这代表了迄今为止报道的通过纳米孔的最快离子流记录。我们观察到 ssDNA 易位事件中短至 200 ns 的瞬态特征，即使在高达 1 MHz 的带宽下，这些瞬态特征也被隐藏。这些特征提供了对分子进出孔的易位动力学的进一步了解。该平台突出了通过集成纳米孔和定制设计的电子器件而实现的高带宽易位测量的优势。
Despite the potential for nanopores to be a platform for high-bandwidth study of single-molecule systems, ionic current measurements through nanopores have been limited in their temporal resolution by noise arising from poorly optimized measurement electronics and large parasitic capacitances in the nanopore membranes. Here, we present a complementary metal-oxide-semiconductor (CMOS) nanopore (CNP) amplifier capable of low noise recordings at an unprecedented 10 MHz bandwidth. When integrated with state-of-the-art solid-state nanopores in silicon nitride membranes, we achieve an SNR of greater than 10 for ssDNA translocations at a measurement bandwidth of 5 MHz, which represents the fastest ion current recordings through nanopores reported to date. We observe transient features in ssDNA translocation events that are as short as 200 ns, which are hidden even at bandwidths as high as 1 MHz. These features offer further insights into the translocation kinetics of molecules entering and exiting the pore. This platform highlights the advantages of high-bandwidth translocation measurements made possible by integrating nanopores and custom-designed electronics.