Neuroprotective effect of the Chinese medicine Tiantai No. 1 and its molecular mechanism in the senescence-accelerated mouse prone 8.
Neuroprotective effect of the Chinese medicine Tiantai No. 1 and its molecular mechanism in the senescence-accelerated mouse prone 8.
复制标题
中药天泰一号对衰老加速小鼠易感8的神经保护作用及其分子机制。
DOI：
10.4103/1673-5374.200813
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发表时间：
2017-02
影响因子：
6.1
通讯作者：
Wu ZZ
中科院分区：
文献类型：
--
作者：
Li YH;Wang XS;Chen XL;Jin Y;Chen HB;Jia XQ;Zhang YF;Wu ZZ
天泰1号是一种中药，主要由天麻、人参、银杏叶按2：1：2的比例组成，溶于纯水，在各种认知障碍的动物模型中具有神经保护作用，但其分子机制尚不清楚。天泰1号以50、100、150 mg/(kg·d)剂量连续灌胃给药8周，观察小鼠Morris水迷宫行为和脑内阿尔茨海默病相关蛋白的表达。天泰1号在水迷宫训练实验中缩短了逃避潜伏期，在空间探测实验中延长了小鼠在目标象限游泳的时间，表明天泰1号改善了SAMP8小鼠的学习记忆能力。免疫组织化学显示，天泰1号恢复了海马区Ki67阳性细胞的增殖能力。此外，服用天泰1号的小鼠星形胶质细胞较少，淀粉样β蛋白和磷酸化tau的积累较少。这些结果表明，天泰1号对SAMP8阿尔茨海默病模型小鼠具有神经保护作用，其作用机制是通过恢复海马神经元数量和增殖潜能，减少星形胶质细胞的浸润，减少淀粉样β蛋白和磷酸化tau的积聚。
Tiantai No. 1, a Chinese medicine predominantly composed of powdered Rhizoma Gastrodiae, Radix Ginseng, and Ginkgo leaf at a ratio of 2:1:2 and dissolved in pure water, is neuroprotective in animal models of various cognitive disorders, but its molecular mechanism remains unclear. We administered Tiantai No. 1 intragastrically to senescence-accelerated mouse prone 8 (SAMP8) mice (a model of Alzheimer's disease) at doses of 50, 100 or 150 mg/kg per day for 8 weeks and evaluated their behavior in the Morris water maze and expression of Alzheimer's disease-related proteins in the brain. Tiantai No. 1 shortened the escape latency in the water maze training trials, and increased swimming time in the target quadrant during the spatial probe test, indicating that Tiantai No. 1 improved learning and memory in SAMP8 mice. Immunohistochemistry revealed that Tiantai No. 1 restored the proliferation potential of Ki67-positive cells in the hippocampus. In addition, mice that had received Tiantai No. 1 had fewer astrocytes, and less accumulation of amyloid-beta and phosphorylated tau. These results suggest that Tiantai No. 1 is neuroprotective in the SAMP8 mouse model of Alzheimer's disease and acts by restoring neuronal number and proliferation potential in the hippocampus, decreasing astrocyte infiltration, and reducing the accumulation of amyloid-beta and phosphorylated tau.