Effect of gefarnate on the ocular surface in squirrel monkeys.
Effect of gefarnate on the ocular surface in squirrel monkeys.
复制标题
吉法酯对松鼠猴眼表的影响。
DOI：
10.1097/00003226-200204000-00011
复制
发表时间：
2002
期刊：
影响因子：
2.8
通讯作者：
Beuerman,RogerW
中科院分区：
文献类型：
--
作者：
Toshida,Hiroshi;Nakata,Katsuhiko;Hamano,Takashi;Nakamura,Masatsugu;Nguyen,Doan;Beuerman,RogerW
目的：研究吉法酯(法尼基香叶基乙酸酯)对轻度碱损伤后灵长类动物眼杯状细胞功能的刺激作用。方法：用直径4 mm的滤纸浸泡0.5%氢氧化钠30秒，在松鼠下眼睑结膜表面造成双侧损伤。每只猴一只眼和对侧眼单独滴入1%吉法林滴眼液，每日6次，每周5天，共4周。伤前和伤后每周进行裂隙灯生物显微镜、眼表印迹细胞学染色、荧光素和孟加拉玫瑰红染色以及粘蛋白免疫印迹检测。结果：局部应用吉法酯与任何不良的眼表反应无关。与赋形剂治疗眼相比，吉法酯治疗眼损伤后杯状细胞再生成明显增多。吉法酯治疗的眼在伤后1周泪液粘蛋白含量显著增加。在伤后3周，吉法酯治疗眼的荧光素染色明显减少，2周时创面的孟加拉玫瑰红染色明显低于赋形剂治疗眼；在其他时间，两组眼的结膜染色无明显差异。结论吉法酯促进结膜损伤后杯状细胞的再生，增加粘蛋白的产生。未发现治疗的不良反应。因此，这种制剂在降低杯状细胞功能的情况下可能很有用。
Purpose.To investigate the ability of gefarnate (geranyl farnesylacetate) to stimulate goblet cell function in the primate eye after a mild alkali injury of the tarsal conjunctiva.Methods.A bilateral injury was created on the conjunctival surface of the lower eye lid of squirrel monkeys by means of a 30-second application of a 4-mm diameter piece of filter paper wetted with 0.5% NaOH. Gefarnate drops (1%) were administered to one eye of each monkey and vehicle alone in the contralateral eye six times a day, 5 days a week for 4 weeks. Slit-lamp biomicroscopy, impression cytology staining of the ocular surface, fluorescein and rose bengal staining, and Western blot for mucin were performed before injury and weekly thereafter. Light microscopy was used to evaluate the lower conjunctiva.Results.Topical application of gefarnate was not associated with any adverse ocular surface effects. Goblet cell repopulation after injury was significantly greater in the gefarnate-treated eyes compared with the vehicle-treated eyes. In the gefarnate-treated eyes, tear mucin content was significantly greater at 1 week after injury. Fluorescein staining was significantly reduced at 3 weeks after injury, and rose bengal staining was significantly reduced in the area of the wound at 2 weeks in the gefarnate-treated eyes compared with the vehicle-treated eyes; at other times, conjunctival staining in the two groups of eyes was not significantly different.Conclusions.Gefarnate promotes goblet cell repopulation and increases mucin production after a conjunctival injury. No adverse affects of the treatment were found. Thus, this agent may be useful in conditions that diminish goblet cell function.