A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4
A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4
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DOI：
10.1016/j.femsec.2005.04.007
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发表时间：
2005-10-01
影响因子：
4.2
通讯作者：
Burns, RG
中科院分区：
文献类型：
--
作者：
Aspray, TJ;Hansen, SK;Burns, RG
以土壤悬浮液为源，在用2，4-二氯苯氧乙酸（2，4-D）（25 μ g/ml）灌溉的流动池中启动微生物群落的发育。通过16 S rRNA基因测序鉴定了该群落的可培养细菌成员，发现它们是假单胞菌属、伯克霍尔德氏菌属、棒状单胞菌属和红球菌属的成员。将2，4-D降解供体菌株恶臭假单胞菌SM 1443（pJP 4：：gfp）接种到含有2天龄生物膜群落的流动池室中。pJP 4：：gfp从供体到细菌群落的转移可检测为GFP荧光细胞，并且使用共聚焦扫描激光显微镜捕获图像（由于染色体定位的lacl（q）阻遏基因的存在，GFP荧光在供体中被阻遏）。在每个小室中可以看到大约5-10个直径为20-40 μ m的接合子小菌落。从流动池系统中分离到一株2，4-D降解转化接合子菌株，属于伯克霍尔德氏菌属。(C)2005年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
A soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-D) (25 mu g ml(-1)). Culturable bacterial members of the community were identified by 16S rRNA gene sequencing and found to be members of the genera Pseudomonas, Burkholderia, Collimonas and Rhodococcus. A 2,4-D degrading donor strain, Pseudomonas putida SM 1443 (pJP4::gfp), was inoculated into flow cell chambers containing 2-day old biofilm communities. Transfer of pJP4::gfp from the donor to the bacterial community was detectable as GFP fluorescing cells and images were captured using confocal scanning laser microscopy (GFP fluorescence was repressed in the donor due to the presence of a chromosomally located lacl(q) repressor gene). Approximately 5-10 transconjugant microcolonies, 20-40 mu m in diameter, could be seen to develop in each chamber. A 2,4-D degrading transconjugant strain was isolated from the flow cell system belonging to the genus Burkholderia. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.