The Effect of Adipose-Derived Stem Cells on Ischemia-Reperfusion Injury: Immunohistochemical and Ultrastructural Evaluation
The Effect of Adipose-Derived Stem Cells on Ischemia-Reperfusion Injury: Immunohistochemical and Ultrastructural Evaluation
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DOI：
10.1097/prs.0b013e3181b17bb4
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发表时间：
2009-09-01
影响因子：
3.6
通讯作者：
Hyakusoku, Hiko
中科院分区：
文献类型：
--
作者：
Uysal, A. Cagri;Mizuno, Hiroshi;Hyakusoku, Hiko
背景资料：再灌注损伤治疗的进展为整形外科医生将这些治疗应用于皮瓣和植入组织创造了机会。作者研究了脂肪干细胞对皮瓣缺血再灌注损伤的直接和间接作用，以确定脂肪干细胞向内皮细胞的体内分化;血管内皮生长因子（VEGF）、转化生长因子-β和成纤维细胞生长因子的水平;和超微结构的变化明显的扫描电子显微镜，以澄清最初的事件和以下级联反应。在20只ICR小鼠的背部上升高两个相同的基于颅骨的随机皮瓣，尺寸为1 x 5 cm。左侧皮瓣被指定为对照，右侧皮瓣被注射脂肪来源的干细胞。结果：对照组和实验组的平均存活皮瓣长度分别为15.2 ± 3.4 mm和24.4 ± 2.9 mm。对照组和实验组的平均存活皮瓣面积分别为12.9 +/- 4.1 mm（2）和21.8 +/- 3.7 mm（2）。观察脂肪干细胞在体内向内皮细胞分化的情况。免疫组化染色，VEGF，转化生长因子-β和成纤维细胞生长因子显示实验组的水平增加。结论：脂肪干细胞对缺血再灌注损伤具有保护作用，其机制主要是通过调节生长因子发挥作用。虽然VEGF是最重要的损伤抑制剂，但在其他生长因子的帮助下，脂肪干细胞增强了整体级联反应。（Plast。重建124：804，2009）。
Background: Advances in the treatment of reperfusion injury have created an opportunity for plastic surgeons to apply these treatments to flaps and implanted tissues. The authors examined the direct and indirect effects of adipose-derived stem cells on ischemia-reperfusion injury on a skin flap model to determine the in vivo differentiation of adipose-derived stem cells to endothelial cells; the levels of vascular endothelial growth factor (VEGF), transforming growth factor-beta, and fibroblast growth factor; and the ultrastructural changes apparent with scanning electron microscopy to clarify the initial events and the following cascades.Methods: Two identical cranial based random flaps with a dimension of 1 x 5 cm were elevated on the dorsums of 20 ICR mice. The left flap was designated as the control and the right flap was injected with adipose-derived stem cells. The flaps were then subjected to 6 hours of ischemia by clamping the pedicle, and then reperfusion.Results: The mean viable flap length in the control and experimental groups was 15.2 +/- 3.4 mm and 24.4 +/- 2.9 mm, respectively. The mean viable flap area in the control and experimental groups was 12.9 +/- 4.1 mm(2) and 21.8 +/- 3.7 mm(2), respectively. The in vivo differentiation of adipose-derived stem cells to endothelial cells was observed. The immunohistochemical stainings, VEGF, transforming growth factor-beta, and fibroblast growth factor revealed increased levels in the experimental groups. Scanning electron microscopy indicated mild injury in the experimental group.Conclusions: The adipose-derived stem cells could prevent ischemia-reperfusion injury, mainly by regulating the growth factors. Although VEGF was the foremost inhibitor of injury, the overall cascade was enhanced by adipose-derived stem cells, with the help of the other growth factors. (Plast. Reconstr. Surg. 124: 804, 2009.)