Directed biosynthesis of peptaibol antibiotics in two Trichoderma strains I.: Fermentation and isolation
Directed biosynthesis of peptaibol antibiotics in two Trichoderma strains I.: Fermentation and isolation
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DOI：
10.7164/antibiotics.51.170
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发表时间：
1998-02-01
影响因子：
3.3
通讯作者：
Bodo, B
中科院分区：
文献类型：
--
作者：
Leclerc, G;Rebuffat, S;Bodo, B
肽抗生素是线性的含α-氨基异丁酸的肽抗生素，其来源于主要是木霉属的土壤真菌，并且通过多酶途径在密切相关的类似物的复杂混合物中生物合成。将氨基酸如α-氨基异丁酸（Aib）、谷氨酸或精氨酸添加到两株木霉（T. harzianum和T. longibrachiatum，已经显示导致天然peptaibol混合物的简化，在每种情况下导致几乎唯一的单一肽的生物合成。令人惊讶的是，无论添加的氨基酸是Aib、Glu还是Arg，所获得的肽都是Aib富集的。在T. harzianum中分离到两个新的富含Aib的肽。此外，在T.长臂菌（longibrachiatum）产生在18位具有谷氨酰胺或谷氨酸的中性和酸性20-残基肽，其增加酸性肽的产生。然而，精氨酸是一种带正电荷的氨基酸，通常不存在于peptaibol序列中，当加入到T.哈茨木
Peptaibols are linear alpha-aminoisobutyric acid-containing peptide antibiotics originating from soil fungi mainly of the genus Trichoderma and biosynthesized in complex mixtures of closely related analogues by a polyenzymatic pathway. Addition of amino acids such as alpha-aminoisobutyric acid (Aib), glutamic acid or arginine, to the fermentation medium of two Trichoderma strains, T. harzianum and T. longibrachiatum, has been shown to result in the simplification of the natural peptaibol mixtures, leading in each case to the almost exclusive biosynthesis of a single peptide. Surprisingly, the obtained peptides are Aib-enriched, whether the added amino acid is Aib, Glu or Arg. By adding Aib to the fermentation medium of T. harzianum, two new Aib-rich peptaibols were isolated. Moreover, adding glutamic acid to the culture medium of T. longibrachiatum, which produces both neutral and acidic 20-residue peptaibols with either glutamine or glutamic acid at position 18, increases the production of the acidic peptides. However, arginine which is a positively charged amino acid generally absent from peptaibol sequences, is not incorporated in trichorzins when added to the fermentation medium of T. harzianum.