Preparation and cell culture behavior of self-assembled monolayers composed of chitohexaose and chitosan hexamer
Preparation and cell culture behavior of self-assembled monolayers composed of chitohexaose and chitosan hexamer
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DOI：
10.1016/j.carbpol.2010.04.015
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发表时间：
2010-08
影响因子：
11.2
通讯作者：
Yuka Yoshiike;S. Yokota;N. Tanaka;T. Kitaoka;H. Wariishi
中科院分区：
文献类型：
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作者：
Yuka Yoshiike;S. Yokota;N. Tanaka;T. Kitaoka;H. Wariishi
用氨基硫脲对壳六糖和壳聚糖六聚体的每个还原端进行位点选择性修饰，然后通过 S-Au 键合将 S 衍生物化学吸附在金基底上。石英晶体微天平分析和X射线光电子能谱表明成功形成了由几丁质和壳聚糖组成的自组装单层（SAM），其糖密度分别为0.39和0.35链nm−2。所制备的 SAM 非常亲水（水接触角为 12-13°）并且在纳米尺度上平坦（RMS≈1.7nm）。小鼠成纤维细胞 (NIH-3T3) 优先粘附在两个 SAM 上，而较少的细胞粘附在疏水性更强、完整的金基质上。固定碳水化合物诱导NIH-3T3细胞良好增殖，而游离糖表面处理几乎没有积极作用。这种水溶性寡糖-SAM的结构设计有望为碳水化合物修饰的生物界面的功能开发提供一种新方法。
Chitohexaose and chitosan hexamer were site-selectively modified at each reducing end with thiosemicarbazide, followed by self-assembly chemisorption of the S-derivatives on a gold substrate via S–Au bonding. Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested successful formation of self-assembled monolayers (SAMs) consisting of chitin and chitosan with sugar densities 0.39 and 0.35 chainsnm−2, respectively. As-prepared SAMs were very hydrophilic (with water contact angles 12–13°) and flat at the nanometer scale (RMS≈1.7nm). Mouse fibroblasts (NIH-3T3) preferentially adhered to both SAMs, while fewer cells were attached to the more hydrophobic, intact gold substrate. The fixed carbohydrates induced good proliferation of NIH-3T3 cells, while surface treatment with free sugars had almost no positive effects. This architectural design of water-soluble oligosaccharide-SAMs is expected to provide a new approach for functional development of carbohydrate-decorated biointerfaces.